ELMOD2对胃癌细胞MGC803恶性生物学行为的影响  

Effect of ELMOD2 on malignant biological behaviors of gastric cancer MGC803 cells

作　　者：乔琳琳 陈亮[1] 边月 孙秀菊[1] QIAO Linlin;CHEN Liang;BIAN Yue;SUN Xiuju(Department of Medical Genetics, School of Life Sciences, Chinese Medical University,Shenyang 110122, Liaoning, China)

机构地区：[1]中国医科大学生命科学学院医学遗传学教研室

出　　处：《中国肿瘤生物治疗杂志》2019年第6期632-637,共6页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.81272717)~~

摘　　要：目的:探讨ELMOD2 过表达对胃癌细胞MGC803 恶性生物学行为的影响,并初步研究其相关的分子机制。方法:将GV141-ELMOD2 表达载体转染人胃癌MGC803 细胞,qPCR 及WB实验检测MGC803 细胞中ELMOD2 mRNA和蛋白的表达,CCK-8 法检测MGC803 细胞增殖能力,流式细胞术检测MGC803 细胞凋亡情况,Transwell 法检测MGC803 细胞迁移能力,WB实验检测细胞中PCNA、BAX 和Bcl-2 以及Vimentin 蛋白的表达。结果:ELMOD2 表达载体转染后,MGC803 细胞中ELMOD2 mRNA和蛋白表达水平均显著提高(P<0.05)。ELMOD2 过表达可使MGC803 细胞增殖、迁移能力显著提高,凋亡率显著降低(P<0.05 或P<0.01);细胞中PCNA、Vimentin、Bcl-2 蛋白水平增高,BAX蛋白水平降低(P<0.05 或P<0.01)。结论:ELMOD2 过表达可促进MGC803 细胞增殖、迁移,抑制细胞凋亡,上述效应可能部分通过提高PCNA、Vimentin、Bcl-2 蛋白水平及降低BAX蛋白水平实现。Objective: To investigate the effect of ELMOD2 over-expression on the malignant biological behaviors of gastric cancer MGC803 cells, and to study its related molecular mechanism. Methods: GV141-ELMOD2 expression vector was transfected into human gastric cancer MGC803 cells. The mRNA and protein expressions of ELMOD2 were detected by Real-time fluorescent quantitative PCR and WB, respectively. The cell proliferation ability was detected by CCK-8 method. Apoptosis rate was detected by flow cytometry.The cell migration ability was detected by Transwell method. The protein expressions of PCNA, BAX and Bcl-2 and Vimentin were detected by WB. Results: After transfection of ELMOD2 expression vector, the mRNA and protein expressions of ELMOD2 were significantly increased in MGC803 cells (P<0.05). Further studies showed that over-expression of ELMOD2 increased the proliferation and migration ability but reduced the apoptosis rate of MGC803 cells significantly (P<0.05 or P<0.01). The protein levels of PCNA,Vimentin and Bcl-2 in MGC803 cells increased, while the protein level of BAX decreased (P<0.05 or P<0.01). Conclusion: Over-expression of ELMOD2 can promote the proliferation and migration of MGC803 cells and inhibit cell apoptosis. These effects may be achieved by increasing the protein level of PCNA, Vimentin and Bcl-2, and reducing the protein level of BAX.

关 键 词：ELMOD2胃癌 MGC803细胞 增殖 凋亡 迁移 

分 类 号：R392.12[医药卫生—免疫学] R735.2[医药卫生—基础医学]