有细胞膜结合能力的四丙烯酰基乙酰氨基葡萄糖的合成与生物安全性评价  

Synthesis and biosafety evaluation of four acryl acetyl glucosamine with cell membrane binding ability

作　　者：王晓辉[1] 高萍[1] 程琳[1] 陈斌[1] 刘伟伟[1] 杨子博 史梦柔 李冬冬[1] Wang Xiaohui;Gao Ping;Cheng Lin;Chen Bin;Liu Weiwei;Yang Zibo;Shi Mengrou;Li Dongdong(Tianjin Institute of Pharmaceutical Science,Tianjin 300020,China)

机构地区：[1]天津市医药科学研究所,300020

出　　处：《国际生物医学工程杂志》2019年第2期115-119,138,共6页International Journal of Biomedical Engineering

摘　　要：目的合成并表征四丙烯酰基乙酰氨基葡萄糖(DA-NAG),测其生物相容性和细胞膜结合性能,为其用于医用自凝集凝胶提供依据。方法通过酯化反应合成DA-NAG,采用高效液相色谱/质谱和核磁共振氢谱对产物进行表征,并对产物进行细胞毒性实验和动物皮下植入实验。采用高效液相色谱检测DA-NAG与小鼠成纤维细胞L929细胞膜的结合性能。结果质谱和氢谱结果均符合DA-NAG的结构特征,该化合物无细胞毒性,日本大耳白兔皮下植入4周后可见DA-NAG有明显降解,且对周围组织刺激不明显。高效液相色谱检测结果显示,DA-NAG与L929细胞膜有结合作用。结论成功合成DA-NAG,且DA-NAG具有很好的细胞相容性和细胞膜结合能力。Objective To synthesize and characterize four acryl acetyl glucosamine (DA-NAG),and to determine its biocompatibility and cell membrane binding properties,so as to provide basis for its application in medical self agglutination gels. Methods DA-NAG was synthesized by esterification reaction. The products were characterized by mass spectrometry and hydrogen spectrum. Cytotoxicity test and subcutaneous implantation test were performed on the synthesized DA-NAG. The binding properties of DA-NAG to mouse fibroblast L929 cell membrane were detected using high performance liquid chromatography (HPLC). Results The characterization of mass spectrum and hydrogen spectrum are consistent with the characteristics of DA-NAG. The product has no cytotoxicity,and the subcutaneous implantation shows that the DA-NAG can be degraded at 4 weeks without obvious stimulation to the surrounding tissues. The result of HPLC shows the binding effect between the DA-NAG and cell membrane. Conclusions DA-NAG is successfully synthesized,and it has good cytocompatibility and binding ability to cell membrane.

关 键 词：细胞膜 四丙烯酰基乙酰氨基葡萄糖 自凝集凝胶 生物安全性 

分 类 号：R318.08[医药卫生—生物医学工程]