水稻粒质量和粒形QTL定位及粒长位点qGL3.2的鉴定  

Mapping of QTL for grain weight and grain shape and identification of qGL3.2 for grain length in rice

作　　者：游佳 谷晗 朱泽 肖世卓 王致远 刘子文 胡曼曼 刘世家[1] 陈亮明[1] 刘喜[1] 田云录[1] 江玲[1] 刘玲珑 YOU Jia;GU Han;ZHU Ze;XIAO Shizhuo;WANG Zhiyuan;LIU Ziwen;HU Manman;LIU Shijia;CHEN Liangming;LIU Xi;TIAN Yunlu;JIANG Ling;LIU Linglong(State Key Laboratory of Crop Genetics and Germplasm Enhancement/Research Center of Jiangsu PlantGene Engineering,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心

出　　处：《南京农业大学学报》2019年第4期612-621,共10页Journal of Nanjing Agricultural University

基　　金：国家重点研发计划项目(2017YFD6100305);江苏省科技支撑计划项目(BE2015355-1);江苏省农业科技自主创新项目[CX(16)1029];国家自然科学基金项目(31571629)

摘　　要：[目的]通过水稻粒质量和粒形相关基因的QTL分析,挖掘普通野生稻(OryzarufipogonGriff.)中优异基因,为高产水稻资源的鉴定与品种改良提供研究基础和创新材料。[方法]以籼稻品种‘9311’为受体亲本,普通野生稻为供体亲本构建的染色体片段置换系群体为研究材料,考察2015、2016和2017年水稻种子千粒质量、粒长、粒宽和长宽比等性状,利用QTLIciMapping4.1软件对控制籽粒大小的QTL进行定位分析。并利用‘9311’与小粒家系Q8衍生的次级F2群体验证第3染色体1个效应明显的QTL(qGL3.2)。[结果]3年共定位到16个QTL,分布于第2、3、6、8和11染色体上,解释遗传变异的4.52%~13.08%。‘9311’与Q8家系衍生的次级F2群体验证了标记Indel3-17和RM3646之间qGL3.2位点的真实性,其对千粒质量、粒宽和长宽比的贡献率分别为33.18%、8.76%和59.92%;对粒长的效应尤为明显,LOD值高达39.29,可解释表型变异的贡献率达61.43%。精细定位和测序分析表明qGL3.2在基因Os03g0407400编码区发生1个C-A的替换,导致蛋白翻译提前终止。对130份种质资源等位变异类型和籽粒长度的分析表明C-A等位变异与粒长高度相关。[结论]qGL3.2位点Os03g0407400基因C-A突变对水稻粒质量和粒形有重要影响,这为该基因的进一步育种利用和分子标记辅助选择提供依据。[Objectives] The objective of this study was to explore the favorable alleles in Oryza rufipogon Griff. and provide research basis and innovative materials for the identification and variety improvement of high-yielding rice resources through QTL analysis of rice grain weight and grain shape-related genes.[Methods] A set of chromosome segment substitution line(CSSL),derived from an indica rice variety‘9311’as the recurrent parent and the Oryza rufipogon Griff. as the donor parent,were used as research materials. Four traits including 1 000-grain weight,grain length,grain width and length-to-width ratio of rice seeds were measured in 2015,2016 and 2017. The QTL IciMapping 4.1 software was employed to detect the QTL controlling the four grain size-related traits. Further,a main QTL( qGL 3.2 )located on chromosome 3 was verified by using an F 2 population derived from the crossing between‘9311’and a small-grain CSSL(named Q8).[Results] A total of 16 QTL were detected on chromosomes 2,3,6,8 and 11 in three years,with phenotypic variation explained(PVE)from 4.52% to 13.08%. The qGL 3.2 locus between the markers Indel3-17 and RM3646 was confirmed by the F 2 population derived from‘9311’and Q8,and its PVE to 1 000-grain weight,grain width and length-to-width ratio were 33.18%, 8.76% and 59.92%,respectively. Markedly,the LOD score of qGL 3.2 to grain length was as high as 39.29,with PVE of 61.43%. Fine mapping and sequencing analysis showed that qGL 3.2 locus had a C-A substitution in the coding region of the gene Os 03g0407400 , resulting in the premature termination of protein translation. Analysis of allelic variation and grain length of 130 germplasm resources showed that C-A variation was highly correlated with grain length.[Conclusions] The C-A mutation in qGL 3.2(Os03g0407400 )has an important effect on grain weight and grain shape of rice,which provides a basis for further breeding and marker-assisted selection.

关 键 词：普通野生稻 染色体片段置换系 QTL 千粒质量 粒长 粒宽 长宽比 

分 类 号：S511.032[农业科学—作物学]