人参皂苷Rg1对大鼠脊髓缺血再灌注损伤中survivin蛋白表达及细胞凋亡的影响  

Effects of ginsenoside Rg1 on survivin protein expression and apoptosis in rats with spinal cord ischemia-reperfusion injury

作　　者：叶劲涛 宋焕瑾[1] 李锋涛[1] 林磊[2] 薛建利[1] 吴昊 程斌[1] YE Jin-tao;SONG Huan-jin;LI Feng-tao;LIN Lei;XUE Jian-li;WU Hao;CHENG Bin(Department of Orthopedics,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004;Department of Orthopedics,Hanzhong Central Hospital,Hanzhong 723000,China)

机构地区：[1]西安交通大学第二附属医院,陕西西安710004 [2]汉中市中心医院,陕西汉中723000

出　　处：《西安交通大学学报（医学版）》2019年第4期646-652,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基金面上项目(No.2014JM2_8157,No.2016JM8129)~~

摘　　要：目的观察人参皂苷Rg1预处理对于大鼠脊髓缺血再灌注损伤后的survivin蛋白表达及细胞凋亡的影响,探讨人参皂苷Rg1对脊髓缺血再灌注损伤后大鼠运动功能恢复的可能机制。方法选取成年健康的SD大鼠120只,构建大鼠脊髓缺血再灌注损伤模型,随机分为假手术组、缺血组、缺血再灌注组及药物组。采用后肢神经运动功能(BBB)评价大鼠后肢神经运动功能;免疫组化法观察survivin蛋白及凋亡诱导因子(apoptosis-inducing factor,AIF)表达;Western blot及RT-PCR方法分析各组survivin及Caspase-9的表达和活性。结果人参皂苷Rg1的干预可以使大鼠后肢神经运动功能评分升高,AIF阳性细胞数减少、survivin蛋白阳性细胞数增多、survivin及Caspase-9的表达减少、神经细胞凋亡减少。结论人参皂苷Rg1可能通过促进survivin蛋白的表达来抑制Caspase-9表达以及降低胞质AIF的水平介导大鼠脊髓缺血再灌注损伤造成的细胞凋亡。Objective To observe the effects of ginsenoside Rg1 pretreatment on the expression of survivin protein and apoptosis after spinal cord ischemia-reperfusion injury (SCII) in rats so as to explore the possible mechanism of ginsenoside Rg1 on motor function recovery after SCII in rats. Methods We selected 120 adult healthy SD rats to construct the model of SCII and randomly divided them into four groups: sham operation group,ischemia group,ischemia-reperfusion group,and drug group. Basso Beattie and Bresnahan score (BBB score) was used to evaluate the motor function of the hind limbs of the rats. The expressions of survivin protein and apoptosis-inducing factor (AIF) was observed by immunohistochemistry. The expression and activity of survivin protein and Caspase-9 in each group were observed and analyzed by Western blot and RT-PCR. Results The intervention of ginsenoside Rg1 could increase the score of the motor function of the rat hind limbs. It could decrease the number of AIF positive cells,but increase the number of survivin protein positive cells. Ginsenoside Rg1 could decrease the expressions of survivin and Caspase-9,and decrease the apoptosis of nerve cells in SCII. Conclusion Ginsenoside Rg1 could inhibit the expression of Caspase-9 by promoting the expression of survivin protein and decrease the apoptosis of rat SCII induced by the level of cytoplasmic AIF.

关 键 词：脊髓缺血再灌注损伤 人参皂苷RG1 细胞凋亡 SURVIVIN蛋白 Caspase-9 凋亡诱导因子 

分 类 号：R651.2[医药卫生—外科学]