锌指蛋白84基因促进宫颈癌细胞增殖的初步研究  

Preliminary study on ZNF84 gene in promoting the proliferation of cervical cancer cells

作　　者：石海燕[1] 胡维维[1] 张鑫[1] 吕晋[1] 杨秀媚[1] 颜菲 SHI Hai-yan;HU Wei-wei;ZHANG Xin;LV Jin;YANG Xiu-mei;YAN Fei(The First People’s Hospital of Foshan,Foshan 528000,China)

机构地区：[1]广东省佛山市第一人民医院

出　　处：《广东医科大学学报》2019年第3期246-250,共5页Journal of Guangdong Medical University

摘　　要：目的探讨锌指蛋白84基因(ZNF84)在宫颈病变组织中的表达及其对肿瘤细胞增殖的影响。方法收集某院2015年1月至2016年5月正常宫颈组织15例和宫颈癌组织45例,应用免疫组织化学法检测组织中ZNF84蛋白的表达;培养宫颈癌Hela细胞和C33a细胞,合成ZNF84RNA干扰序列siZNF84,并将siZNF84转染宫颈癌细胞检测ZNF84基因的表达;CCK-8法检测siZNF84对宫颈癌细胞增殖的影响。结果ZNF84在宫颈正常组织不表达,在宫颈癌组织的表达率为77.8%(35/45),差异有统计学意义(P<0.01)。ZNF84蛋白表达与肿瘤大小、HPV感染有关(P<0.01或0.05)。siZNF84显著下调了ZNF84的mRNA表达,抑制了宫颈癌Hela细胞和C33a细胞的体外增殖能力(P<0.05)。结论ZNF84在宫颈癌组织中表达,与肿瘤大小、HPV感染有关,能够促进肿瘤细胞增殖,可能对宫颈癌的发生、发展有一定的影响。Objective To investigate the expression of zinc finger protein 84 (ZNF84) in cervical lesion tissues and the effect of ZNF84 in promoting the proliferation of cancer cells. Methods Fifteen cases of normal cervical tissues and 45 cases of cervical cancer tissues in a hospital from January 2015 to May 2016 were collected. The expression of ZNF84 protein in cervical cancer tissues was detected by immunohistochemistry. Hela cells and C33a cells were cultured. ZNF84 RNA interference sequence, siZNF84, was synthesized. The siZNF84 was transfected into cervical cancer cells to detect the expression of ZNF84 gene. The effect of siZNF84 in promoting the proliferation of cervical cancer cells was detected by CCK-8 method. Results ZNF84 was not expressed in normal cervical tissues, but had an expression rate of 77.8%(35/45) in cervical cancer tissues, and the difference was statistically significant (P<0.01). The expression of ZNF84 protein was correlated to tumor size and HPV infection (P<0.01 or 0.05). SiZNF84 significantly downregulated the mRNA expression of ZNF84 and significantly inhibited the in vitro proliferation capacity of cervical cancer Hela cells and C33a cells (P<0.05). Conclusion The ZNF84 is expressed in cervical cancer tissues, which is correlated to tumor size and HPV infection. ZNF84 can promote the proliferation of cancer cells and may have certain effect on the incidence and development of cervical cancer.

关 键 词：宫颈癌 ZNF84 增殖 免疫组化 

分 类 号：R446.8[医药卫生—诊断学]