药用狗牙花的单萜吲哚生物碱类成分  

Studies on the monoterpenoid indole alkaloids constituents of Ervatamia officinalis

作　　者：程春雷 袁孟菲 谢静 张建 张晓琦[2] 叶文才[2] CHENG Chunlei;YUAN Mengfei;XIE Jing;ZHANG Jian;ZHANG Xiaoqi;YE Wencai(Shandong Institute for Food and Drug Control,Jinan 250101,China;College of Pharmacy,Guangdong Provincial Engineering Research Center for Modernization of TCM,Jinan University,Guangzhou 510632,China)

机构地区：[1]山东省食品药品检验研究院,山东济南250101 [2]暨南大学药学院,广东省现代工程技术研究中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2019年第4期288-294,共7页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(U1801287; 81803377);国家重点研发计划项目(2017YFC1703802);广东省科技计划项目(2018B020207008)

摘　　要：目的:研究药用狗牙花(Ervatamia officinalis)中的生物碱类成分.方法:采用硅胶、Sephadex LH-20、C 18键合硅胶、HPLC制备等色谱方法进行分离纯化,用波谱学和化学方法鉴定化学结构.结果:用体积分数为95%的乙醇提取药用狗牙花枝叶,分离鉴定了9个单萜吲哚生物碱化合物,分别为lirofoline A(1)、lirofoline B(2)、lochvinerine(3)、洛柯碱(4)、去甲马枯素B(5)、16-表-去甲马枯素B(6)、16-表-老刺木碱(7)、老刺木碱(8)及14,15-didehydro-16-epi-vincamine(9).结论:化合物1~7为首次从药用狗牙花中分离得到,并得到化合物1和3的单晶X线数据.Objective:To isolate and identify the alkaloid constituents from the leaves and twigs of Ervatamia officinalis Tsiang.Methods:Using of silica gel,Sephadex LH-20,Octadecylsilyl(ODS)and preparative HPLC chromatographies to isolate the total alkaloids of E.officinalis.Their structure was identified by NMR,ESI-MS,X-ray analysis and chemical assays.Results:Nine monoterpenoid indole alkaloids were isolated from total alkaloids in 95%ethanol extract and identified as lirofoline A(1),lirofoline B(2),lochvinerine(3),lochnerine(4),normacusine B(5),16-epi-normacusine B(6),16-epi-vobasine(7),vobasine(8)and 14,15-didehydro-16-epi-vincamine(9).Conclusion:Compounds 1~7 were isolated from this plant for the first time.The single-crystal X-ray diffraction data of compounds 1 and 3 are first reported.

关 键 词：药用狗牙花 夹竹桃科 单萜吲哚生物碱 单晶结构 

分 类 号：R932[医药卫生—生药学]