SerpinA3对胃癌细胞MGC803的增殖行为研究  

Study of SerpinA3 on proliferation in Gastric cancer cell line MGC803

作　　者：罗文[1] 李群芳[1] 严超[1] 萧赪 LUO Wen;LI Qun-fang;YAN Chao(Department of Internal Medicine,Hunan Vocational and Technical College of Environmental Biology, Hengyang 421001, China)

机构地区：[1]湖南环境生物职业技术学院内科教研室

出　　处：《中国处方药》2019年第7期45-47,共3页Journal of China Prescription Drug

基　　金：湖南省教育厅资助课题(16C0557)

摘　　要：目的分析SerpinA3高表达对胃癌MGC803细胞增殖行为的影响。方法首先根据试剂操作说明成功构建pcDNA3.1-SerpinA3真核表达载体,分别采用pcDNA3.1-SerpinA3、pcDNA3.1转染人胃癌MGC803细胞系,经G418筛选稳定表达。实验分为三组,即空白对照组(MGC803正常培养)、pcDNA3.1转染MGC803组、pcDNA3.1-SerpinA3转染MGC803组;MTT法检测细胞增殖率、平板克隆形成和软琼脂集落形成实验比较克隆形成和集落形成率、流式细胞术检测凋亡率。结果pcDNA3.1-SerpinA3组细胞增长率显著降低,克隆形成和集落形成率减少,凋亡率增加,差异有统计学意义(P<0.05)。结论SerpinA3可促进胃癌细胞增殖,抑制凋亡,可能参与肿瘤的发生,有望成为诊断胃癌的敏感标志物。Objective To analyze high expression of SerpinA3 on the proliferation of gastric cancer MGC803 cells. Methods Firstly, the eukaryotic expression vectors of pcDNA3.1-SerpinA3 were successfully constructed according to instructions of reagent operation. pCDNA3.1-SerpinA3 and pcDNA3.1 were transfected into human gastric cancer MGC803 cell lines respectively. The stable expression was screened by G418. The MGC803 cells were divided into 3 groups: blank control group( normal culture of MGC803), pcDNA 3.1 transfecting MGC803 group, pcDNA 3.1-SerpinA3 transfecting MGC803 group, then to detect cell proliferation rate with MTT assay, plate cloning and soft agar colony formation rates, and cell apoptosis rate with flow cytometry. Results The cell proliferation rate in pcDNA 3.1-SerpinA3 transfecting group was significantly lower, plate cloning and soft agar colony formation rates were less, apoptosis rate was higher than the other 2 groups( P < 0.05). Conclusion SerpinA3 can promote proliferation of gastric cancer cells, inhibit apoptosis, may participate in occurrence of tumors, which is expected to become a sensitive marker for gastric cancer diagnosis.

关 键 词：SerpinA3蛋白 胃癌 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]