多重实时荧光PCR在诊断学龄前期CAP中应用价值  

Application Value of Multiple Real-time Fluorescent PCR in the Diagnosis of Pre-school CAP

作　　者：樊旭成[1] 薛娜[1] 韩志国[1] 高枫[1] FAN Xu-cheng;XUE Na;HAN Zhi-guo;GAO Feng(Department of Infectious Disease Control,Urumqi Center for Disease Control and Prevention, Urumqi 830026,Xinjiang,China)

机构地区：[1]乌鲁木齐市疾病预防控制中心传染病防治科

出　　处：《医学信息》2019年第13期160-161,共2页Journal of Medical Information

基　　金：乌鲁木齐市科学技术计划重点实验室项目(编号:H171313001)

摘　　要：目的探讨多重实时荧光聚合酶链式反应(PCR)在学龄前期社区获得性肺炎(CAP)诊断中的应用价值。方法筛选2018年1月~12月到乌鲁木齐市某社区服务门诊就诊的206例CAP患儿,给予常规采血检测降钙素原(PCT)和C-反应蛋白(CRP)水平,同时所有患儿均接受多重实时荧光PCR检测感染病原体并比较。结果常规采血方法检测结果呈阳性有116例患儿,占56.31%;采用多重实时荧光PCR检测结果呈阳性共有201例患儿,占97.57%。采用多重实时荧光PCR检测的结果阳性率高于常规采血检测,差异具有统计学意义(P<0.05)。结论多重实时荧光PCR在学龄前期社区获得性感染中的应用效果良好,具有很高的灵敏度,方便快捷,能够提供较高价值的诊疗参考。Objective To investigate the value of multiple real-time fluorescent polymerase chain reaction (PCR) in the diagnosis of pre-school community-acquired pneumonia(CAP). Methods A total of 206 children with CAP who were admitted to a community outpatient clinic in Urumqi from January to December 2018 were enrolled. The routine blood sampling was performed to detect procalcitonin (PCT) and C-reactive protein (CRP) levels. Multiple real-time fluorescent PCR was performed to detect infectious pathogens and compare them. Results The results of routine blood collection were positive in 116 cases, accounting for 56.31%. The results of multiple real-time fluorescent PCR were positive in 201 cases, accounting for 97.57%. The positive rate of multiple real-time fluorescent PCR was higher than that of conventional blood sampling,the difference was statistically significant (P<0.05). Conclusion Multiple real-time fluorescent PCR has a good effect in pre-school community acquired infections. It has high sensitivity, is convenient and fast, and can provide high-value diagnosis and treatment reference.

关 键 词：多重实时荧光PCR 学龄前期 社区获得性感染 

分 类 号：R446[医药卫生—诊断学]