槿柏洗剂抑菌效果评价及其对糠秕马拉色菌感染豚鼠模型外周血中Th1/Th2相关因子的影响  

Evaluation of antibacteria effect of Jinbai Lotion and its effects to Th1/Th2 relevant factors of peripheral blood of guinea pig model infected with malassezia

作　　者：胡博 张丰川[1] 李元文[1] 蔡玲玲[1] 姜颖娟[1] 姜晓媛 付蓉[1] HU Bo;ZHANG Fengchuan;LI Yuan-wen(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学东方医院皮肤性病科

出　　处：《环球中医药》2019年第6期831-835,共5页Global Traditional Chinese Medicine

基　　金：北京中医药大学基本科研业务项目(中青年教师类)(2016-JYB-JSMS-048)

摘　　要：目的评价槿柏洗剂的抑菌效果,通过检测干预前后模型外周血中Th1/Th2平衡状态,了解马拉色菌感染对系统免疫的影响,探讨槿柏洗剂治疗马拉色菌感染性疾病的可能作用机制。方法ELISA法检测槿柏洗剂组、酮康唑洗剂组、模型组与空白组豚鼠外周血中γ-干扰素、白介素-4、白介素-2、白介素-10含量。结果模型组白介素-2、γ-干扰素水平较空白组、槿柏洗剂组、酮康唑组显著降低。槿柏洗剂组与酮康唑组之间差异无统计学意义(P>0.05)。模型组豚鼠血清中白介素-4、白介素-10含量较空白组、槿柏洗剂组、酮康唑组明显升高(P<0.05)。槿柏洗剂组与酮康唑组之间差异无统计学意义(P>0.05)。结论糠秕马拉色菌豚鼠感染模型存在Th1/Th2漂移现象,槿柏洗剂可能通过调节Th1/Th2细胞平衡发挥抗炎、抑菌效应。Objective To evaluate of the antibacterial effect of Jinbai Lotion,to investigate the effects of malassezia infection on systemic immunity by detecting the balance of Th1/Th2 in the peripheral blood of the model before and after intervention,and to explore the possible mechanism of treating malasse- zia infectious diseases with Jinbai Lotion. Methods The levels of INF-γ,IL-4,IL-2 and IL-10 in the pe- ripheral blood of Jinbai Lotion group,ketoconazole Lotion group,model group and blank group of guinea pigs were detected by ELISA method. Results In the model group,IL-2 and INF-γ levels decreased sig- nificantly,compared with the blank group,Jinbai Lotion group and Ketoconazole Lotion group. There was no statistical significance ( P >0. 05) between Jinbai Lotion group and Ketoconazole Lotion group. The IL-4 and IL-10 levels of serum in guinea pig of the model group was higher than that of the blank group and the Ketoconazole group increased significantly ( P <0. 05). There was no statistical significance ( P > 0. 05) between Jinbai Lotion group and Ketoconazole Lotion group. Conclusion The model of guinea pig infected with malassezia has Th1/Th2 drifting phenomenon. Jinbai Lotion might exert anti-inflammatory and anti- bacterial effects by regulating Th1/Th2 cell balance.

关 键 词：脂溢性皮炎 马拉色菌 中医药治疗 

分 类 号：R285.5[医药卫生—中药学]