三七总皂苷对肝癌HepG2细胞增殖、侵袭、凋亡及酶活性的影响  

Effects of panax notoginseng saponins on proliferation,invasion,apoptosis,enzyme activity of liver cancercell lines HepG2

作　　者：刘静[1] 张微[1] 张谦[1] Liu Jing;Zhang Wei;Zhang Qian(Department of Pharmacy,Affiliated Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010110,China)

机构地区：[1]内蒙古医科大学新药安全评价研究中心

出　　处：《北方药学》2019年第7期127-129,共3页Journal of North Pharmacy

基　　金：内蒙古医科大学青年创新基金项目(项目编号:YKD2016QNCX017)

摘　　要：目的:探讨三七总皂苷(PNS)对肝癌HepG2细胞增殖、迁移、酶活性及凋亡的影响。方法:应用不同浓度的PNS分别作用于HepG2细胞24、48、72h,采用MTT法测定细胞增殖率,Transwell实验检测肝癌细胞侵袭能力,流式细胞仪检测细胞凋亡水平,全自动生化分析仪检测肝癌细胞酶活性的改变。结果:PNS能抑制肝癌HepG2细胞的增殖和迁移,降低肝癌细胞酶活性,促进肝癌细胞的凋亡。Objective:To investigate the effects of panax notoginseng saponins (PNS)on the cell proliferation,invasion,apoptosis and enzyme activity of liver cancercell lines HepG2. Methods:HepG2 cells were treated with different density of PNS for 24,48,72 h,Cell proliferation rate was detected by MTT assay;cell invasion ability was detected by Transwell method;the level of apoptosis was detected by flow cytometry,and the change of enzyme activity of liver cancer cells was detected by automatic biochemical analyzer. Conclusion: PNS could inhibit the proliferation and invasion of HepG2 cells,reduce the enzyme activity of hepatoma cells,and promote the apoptosis of hepatoma cells.

关 键 词：三七总皂苷 肝癌 增殖 侵袭 凋亡 酶活性 

分 类 号：R286[医药卫生—中药学]