枇杷叶不同成分抗氧化和止咳祛痰活性研究  

Research on antioxidant and antitussive and expectorant activity of different components from loquat leaves

作　　者：黄素华[1,2] 陈彤 邱丰艳[1,2] 洪燕萍 HUANG Su-hua;CHEN Tong;QIU Feng-yan;HONG Yan-ping(College of Life Science,Longyan University,Longyan 364000,Fujian,China;Fujian Provincial Key Laboratory for Biotechnology and Preventive Veterinary Medicine,Longyan 364000,Fujian,China)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364000 [2]福建省生物技术与预防兽医学重点实验室,福建龙岩364000

出　　处：《湖北农业科学》2019年第12期138-140,146,共4页Hubei Agricultural Sciences

基　　金：福建省科技计划重点项目(2012N0019);福建省自然科学基金项目(2012D100);福建省教育厅项目(JB12204)

摘　　要：采用分光光度法测定枇杷[Eriobotryajaponica(Thunb.)Lindl.]叶提取物中的黄酮、多酚、总萜及其含量;采用DPPH和FRAP法检测其抗氧化活性;采用氨水引咳法和气管酚红排泌法研究其止咳祛痰活性,并分析其成分与抗氧化、止咳祛痰活性的相关性。结果表明,6种枇杷叶提取物均具有抗氧化活性,其中黄酮抗氧化活性显著高于三萜酸,多糖抗氧化活性较差,提取物的抗氧化活性与黄酮和多酚含量显著相关;6种提取物均具有止咳祛痰活性,黄酮和三萜酸的效果相近,多糖较差,成分含量与活性相关性分析显示,黄酮能减少咳嗽次数,总萜能延长咳嗽潜伏期,且有一定的祛痰作用。枇杷叶中黄酮、多酚可能是抗氧化的重要成分,黄酮与止咳祛痰活性相关,通过精制富集有效成分,能提高相关活性。Determination of flavonoid, polyphenol, total terpene and their contents in loquat[Eriobotrya japonica (Thunb.) Lindl.] leaf extracts by spectrophotometry, DPPH and FRAP method were used to determine the antioxidant activity, cough model induced by ammonia liquor in mice to observe the antitussive effects,the method of phenol red execretion in mice was used to investigate expectorant effects,and the correlation of composition and the activity were analyzed. Results showed that the loquat leaf extracts had certain antioxidant activity, the flavonoids were significant better than triterpenoid acid, the polysaccharides were weaker, and the antioxidant activity related to the content of flavonoids and total polyphenols. All the 6 kinds of loquat leaf extract had certain antitussis and expectorant activity, the flavonoids and triterpenoid acid were better than the polysaccharides, the times of cough and the content of flavonoid showed significantly negative correlation, the saponins had certain dosage-effect relation with prolonged the incubation period of cough and expectorable activity. The extracts of loquat leaf had antioxidation and antitussis and expectorant activity, flavonoids and polyphenols could be important part of the antioxidant component, flavonoids and saponins were associated with antitussis and expectorant activity. Through the refined, the effective components can be enriched and the relevant activity can be improved.

关 键 词：枇杷[Eriobotryajaponica(Thunb.)Lindl.]叶提取物 抗氧化 止咳祛痰 黄酮 多酚 总萜 相关性 

分 类 号：S667.3[农业科学—果树学]