微小RNA-155和CD4^+调节性T细胞与冠状动脉不稳定斑块的关系  

Relationship between microRNA-155,CD4^+ regulatory T cells and unstable coronary plaque in patients with coronary artery disease

作　　者：贾敏[1] 刘震[2] 罗义[2] 雷晓明[2] 杨阳[2] 陈平安[2] Jia Min;Liu Zhen;Luo Yi;Lei Xiaoming;Yang Yang;Chen Pingan(General Medicine Ward, Guangzhou Chest Hospital, Guangzhou 510095, China;Department of Cardiology, Guangzhou First People s Hospital, Guangzhou 510180, China)

机构地区：[1]广州市胸科医院综合内科,510095 [2]广州市第一人民医院心内科,510180

出　　处：《中国心血管杂志》2019年第3期209-213,共5页Chinese Journal of Cardiovascular Medicine

基　　金：广州市医药卫生科技项目(20171A011266)~~

摘　　要：目的探讨循环微小RNA-155(miR-155)和外周血CD4^+调节性T细胞(Treg)在冠心病(CAD)患者的表达水平及与冠状动脉不稳定斑块的关系。方法连续入选2016年1月至2018年1月在广州市第一人民医院心内科住院的CAD患者120例,均接受冠状动脉造影和血管内超声检查,通过虚拟组织学技术判断患者冠状动脉内是否具有不稳定斑块,并将患者分为稳定斑块组(SP组)50例和不稳定斑块组(UP组)70例,另外选择同期在体检中心进行健康体检的50名健康者作为对照组。采用实时荧光定量聚合酶链式反应技术测定研究对象的静脉血浆miR-155的相对表达量,并用流式细胞仪采用直接免疫荧光法检测静脉血浆CD4^+CD25+Foxp3+Treg细胞占CD4^+Treg细胞的比例。结果UP组患者的血浆miR-155相对表达量显著低于SP组和对照组(0.57±0.10比0.71±0.09和0.83±0.11,P<0.05);UP组患者的CD4^+CD25+Foxp3+Treg细胞占CD4^+Treg细胞的比例明显低于SP组和对照组(5.92±1.34比8.05±1.39和12.68±1.56,P<0.01)。UP组CAD患者的miR-155相对表达量与CD4^+CD25+Foxp3+Treg细胞水平呈正相关(r=0.476,P=0.013)。多因素logistic回归分析显示,血浆miR-155和CD4^+CD25+Foxp3+Treg细胞水平均是CAD患者斑块稳定的独立保护因子(OR=0.662,95%CI:0.472~0.819,P=0.011;OR=0.502,95%CI:0.376~0.765,P=0.019)。结论MiR-155通过调节CD4^+Treg细胞表达和功能,可能是影响冠状动脉斑块稳定性的重要机制之一。Objective To investigate the relationship between microRNA-155 (miR-155) and CD4^+ regulatory T cells (Treg) and unstable coronary plaque in patients with coronary artery disease. Methods A total of 120 CAD patients with CAD who were hospitalized in the department of cardiology in Guangzhou First People s Hospital from January 2016 to January 2018 were enrolled. All patients underwent coronary angiography and intravascular ultrasound (IVUS) examination. IVUS-virtual histology technique was applied to detect the unstable plague (UP) in patients and direct immunofluorescence method was used to measure the percentage of CD4^+CD25 +Foxp3 + Treg cells in CD4^+ Treg cells by flow cytometry. Plasma miR-155 of patients were measured by the methods of quantitative realtime fluorescent Polymerase Chain Reaction (qRT-PCR). Results The levels of plasma miR-155 and CD4^+CD25 +Foxp3 + Treg cells in patients of UP group were significantly lower than patients in stable plague group and control group (0.57±0.10 vs. 0.71±0.09 and 0.83±0.11, P <0.05;5.92±1.34 vs. 8.05±1.39 and 12.68±1.56, P <0.01). The levels of plasma miR-155 were positively correlated with CD4^+CD25 +Foxp3 + Treg cells in patients of UP group ( r=0.476, P =0.013). Multivariate logistic regression analysis showed that plasma miR-155 and CD4^+CD25 +Foxp3 + Treg cells both were strong protective factors for coronary plague in CAD patients ( OR=0.662, 95%CI: 0.472-0.819, P=0.011;OR=0.502, 95%CI: 0.376-0.765, P =0.019). Conclusions MiR-155 regulated the expression and function of the CD4^+ Treg cells, which may be one important mechanisms in affecting the coronary plaque stability.

关 键 词：微小RNA 调节性T细胞 动脉粥样硬化 冠状动脉疾病 

分 类 号：R541.4[医药卫生—心血管疾病]