烧伤大鼠血清对血管内皮细胞通透性的影响  

Effect of burned-rat serum on the permeability of vascular endothelial cells

作　　者：段声梁 胡子健 孟承颖 黄海良 蒋智永 蒋薇 王欢[1] 余又新[1] 孙业祥[1] 方林森[1] 胡德林[1] Duan Shengliang;Hu Zijian;Meng Chengying(Dept of Burn,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Clinical Medicine,First Clinical Medical College,Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院烧伤科,合肥230022 [2]安徽医科大学第一临床医学院,合肥230022 [3]安徽医科大学基础医学院生物化学与分子生物学教研室,合肥230022

出　　处：《安徽医科大学学报》2019年第6期930-933,共4页Acta Universitatis Medicinalis Anhui

基　　金：2015年度第二批安徽省自然科学基金项目(编号:1508085SMH231);安徽省2018年度重点研究与开发计划项目(编号:1804h08020230)

摘　　要：目的探讨烧伤大鼠血清对血管内皮细胞(EC)通透性的影响及作用机制。方法将原代培养的大鼠主动脉EC根据是否应用烧伤血清干预分为正常组和血清干预组,血清干预组应用烧伤12h大鼠血清干预大鼠主动脉内皮细胞24h,应用细胞通透性实验检测单层细胞膜通透性变化,实时荧光定量PCR(RT-PCR)和Western blot分别检测细胞通透性相关基因内皮素-1(ET-1)、内皮因子受体A(ETA)、ETB和带状闭合蛋白1(ZO-1)的mRNA和蛋白表达的变化。结果与正常组相比,烧伤大鼠血清干预组EC通透性系数明显升高(P<0.01),血清干预组ET-1、ETA和ETB的mRNA和蛋白表达均显著升高(P<0.01)、ZO-1的mRNA和蛋白表达明显降低(P<0.01)。结论烧伤大鼠血清可引起血管EC通透性的增加,通透性增加可能与ET-1、ET-A、ET-B表达升高和ZO-1表达降低有关。Objective To investigate the effect of burned-rat serum on the vascular permeability and its mechanism. Methods Primary cultured rat aortic endothelial cells were divided into normal group and serum intervention group according to whether or not to apply burn serum intervention,serum intervention group was used to treat rat aortic endothelial cells for 24 h with burned 12 h serum.Cell permeability test was used to detect changes in monolayer cell membrane permeability.The expressions of the mRNA and protein of cell permeability-related genes ET-1,ETA,ETB and ZO-1 were detected by Real-time PCR and Western blot respectively. Results Compared with the normal group,the cell permeability coefficient in serum intervention group was significantly increased( P <0.01),the expressions of the mRNA and protein of cell permeability-related genes ET-1,ETA and ETB in serum intervention group were significantly increased( P <0.01),and the expression of the mRNA and protein of ZO-1 in serum intervention group was significantly decreased( P <0.01). Conclusion The serum of burned rats can increase the permeability of vascular endothelial cells,and the increase of permeability may be related to the increased expression of ET-1,ET-A,ET-B and the decrease of ZO-1 expression.

关 键 词：烧伤 内皮细胞 血管通透性 ET-1 ZO-1 

分 类 号：R644[医药卫生—外科学]