小鼠骨骼肌挫伤修复过程中周细胞数量变化及其与损伤时间相关性  

Relationship between Changes of Pericyte Number and Wound Age during Repair of Skeletal Muscle Contusion in Mice

作　　者：温书恒 田志岭 张淼 张孟周 王帅 陈京伟 孙英富 王昌亮 赵锐 官大威 WEN Shu-heng;TIAN Zhi-ling;ZHANG Miao;ZHANG Meng-zhou;WANG Shuai;CHEN Jing-Wei;SUN Ying-fu;WANG Chang-liang;ZHAO Rui;GUAN Da-wei(Center of Medico-legal Investigation,School of Forensic Medicine,China Medical University,Shenyang110122,China;Collaborative Laboratory of Intelligentized Forensic Science,Shenyang 110122,China;Shanghai Key Laboratory of Forensic Medicine,Shanghai Forensic Service Platform,Academy of ForensicScience,Shanghai 200063,China;Liaoning Provincial People’s Procuratorate,Shenyang 110033,China)

机构地区：[1]中国医科大学法医学院法医司法鉴定中心,辽宁沈阳110013 [2]辽宁省智慧检务创新研究院智慧司法鉴定联合实验室,辽宁沈阳110122 [3]司法鉴定科学研究院上海市法医学重点实验室上海司法鉴定专业技术服务平台,上海200063 [4]辽宁省人民检察院,辽宁沈阳110033

出　　处：《法医学杂志》2019年第3期267-272,279,共7页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81273342,81671862,81772023,81871529,81801874);国家重点研发计划子课题资助项目(2018YFC0807204);中国工程院重大咨询研究子课题资助项目(2016-XZ-21-03)

摘　　要：目的观察小鼠骨骼肌挫伤后不同时间段周细胞数量变化规律,并探讨其在损伤时间推断中的作用。方法制作小鼠腓肠肌挫伤模型,应用免疫荧光染色法对小鼠骨骼肌挫伤后1、3、5、7、9、14、28d的周细胞形态及数量进行检测。结果与正常骨骼肌周细胞细长形态相比,挫伤区周细胞体积增大、形态变圆,胞核呈圆形,其中部分周细胞表达卫星细胞标记物配对盒转录因子(paired-box transcription factor,Pax7)或生肌决定因子(myoblast determination,MyoD) 1。挫伤后骨骼肌周细胞数量变化具有时间规律性,并随损伤时间延长呈单峰性分布。挫伤中心区周细胞数量于挫伤后5d达到峰值,挫伤周边区周细胞数量在挫伤后5d和7d达到峰值。结论小鼠骨骼肌挫伤后,挫伤区周细胞数量变化具有一定的时间规律性,提示其可能作为肌肉损伤时间推断的一个参考指标,并参与骨骼肌损伤后的肌肉修复再生过程。Objective To observe the change pattern of pericyte number at different time periods after mice skeletal muscle contusion and discuss its role in wound age estimation. Methods A mice gastrocnemius muscle contusion model was established. The form and number changes of pericytes at 1, 3, 5, 7, 9, 14, and 28 d post-injury were detected by multiple immunofluorescence staining. Results Compared with the slender shape of pericytes in normal skeletal muscles, pericytes in the contusion area had increased volume, rounder form and a round nuclei. Part of pericytes were found to express satellite cell markers paired-box transcription factor (Pax7) or myoblast determination 1 (MyoD1). The changes of pericyte number in skeletal muscles after contusion were time-dependant, and showed unimodal distribution with the extension of wound age. In the central contusion area, the number of pericytes peaked at 5 d post-injury while in the peripheral contusion area, the number of pericytes peaked at 5 d and 7 d post-injury. Conclusion The number of pericytes in contusion area varies time-dependently after skeletal muscle contusion in mice and might be a reference index for muscle wound age estimation, and is involved in the repair and regeneration of skeletal muscle injury.

关 键 词：法医病理学 挫伤 卫星细胞 骨骼肌 周细胞 损伤时间推断 小鼠 

分 类 号：DF795.1[医药卫生—法医学]