一次性信号放大电化学阻抗RNA传感器研究  

Study on a disposable signal amplification electrochemical impedance RNA sensor

作　　者：张永花[1] 王玉芳[1] ZHANG Yonghua;WANG Yufang(College of Chemistry and Chemical Engineering,Henan Key Laboratory of Function-Oriented PorousMaterials,Luoyang Normal University,Luoyang 471934,Henan,China)

机构地区：[1]洛阳师范学院化学化工学院功能导向多孔材料重点实验室

出　　处：《化学研究》2019年第2期140-146,共7页Chemical Research

基　　金：河南省教育厅项目(15A150062)

摘　　要：以自制的丝网印刷碳电极(SPCE)为基体电极,利用DNA四面体纳米探针和酶催化信号放大构建了一个一次性电化学阻抗型RNA传感器.固定在AuNPs修饰的SPCE表面的DNA四面体结构能确保DNA探针具有可控的密度和方向,结合辣根过氧化物酶(HRP)催化H2O2氧化4-氯-1-萘酚(CN)的反应,生成不溶物沉积在电极表面,有效地放大电化学阻抗信号,实现了miRNA的高灵敏阻抗测定.检测限可以低至1.0pmol/L,阻抗值和miRNA-141浓度的对数在3.0~1000pmol/L之间具有良好的定量关系.A disposable electrochemical impedance RNA sensor was fabricated based on homemade screen printed carbon electrode (SPCE) and enzyme-catalyzed signal amplification reaction.The SPCE was modified with AuNPs through electrodeposition and subsequently functionalized with-SH of the DNA nanostructure,which can ensure that the DNA probe has a controllable density and direction.Hydrogen peroxide (HRP) can catalyze oxidation reaction of H2O2 and 4-chloro-1-naphthol (CN) to produce an insoluble precipitate accumulated on the electrode surface,which caused the electron-transfer resistance of the sensor increased greatly.According to the above,signal amplification detection of the target microRNA can be carried out.The proposed method could detect target miRNA at a linear range from 3.0 pmol/L to 1 000 pmol/L,with a detection limit of 1.0 pmol/L.

关 键 词：丝网印刷碳电极 信号放大 电化学阻抗 RNA传感器 

分 类 号：O657.1[理学—分析化学]