针刺对自发性高血压大鼠心肌ANP、ACE2表达和Ang(1-7)/AngⅡ比值的影响  

Effect of acupuncture on the expression of ANP, ACE2 and Ang(1-7)/AngⅡ ratio in heart tissue of spontaneously hypertensive rats

作　　者：纪智[1] 袁静云 王紫娟 张跃 梁靖蓉 吴娇娟 刘清国[1] JI Zhi;YUAN Jingyun;WANG Zijuan;ZHANG Yue;LIANG Jingrong;WU Jiaojuan;LIU Qingguo(School of Acupuncture and Massage, Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学针灸推拿学院

出　　处：《中国医药导报》2019年第17期4-8,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81373727、81574069、81774413);北京市自然科学基金资助项目(7162121)

摘　　要：目的通过观察自发性高血压大鼠(SHR)心肌组织结构和肾素-血管紧张素-醛固酮系统(RAS)作用因子表达的变化,探究针刺对高血压心肌肥厚的防护机制。方法将10只WKY大鼠作为空白组,将20只SHR按照随机数字表法分为模型组和针刺组,每组各10只。所有大鼠均为10周龄SPF级雄性大鼠。适应性饲养1周后,针刺组大鼠用毫针于“百会”和双“太冲”直刺,每天1次,连续干预28 d。模型组和空白组不做针刺干预。于实验第1、7、14、21、28天针刺前测量血压值。实验结束后称取大鼠全心及左心室重量,并计算心脏重量指数(HWI)和左心室重量指数(LVWI)。应用HE和Masson染色法观察心肌组织形态,并通过Real-tiem PCR、Western blot、酶联免疫吸附测定(ELISA)等技术检测各组大鼠心肌组织中心房钠尿肽(ANP)、血管紧张素转换酶2(ACE2)、血管紧张素1-7[Ang(1-7)]和AngⅡ的表达,并计算Ang(1-7)/AngⅡ。结果与空白组比较,模型组大鼠收缩压(SBP)、HWI、LVWI显著升高(P < 0.01)。与模型组比较,针刺组大鼠SBP、HWI、LVWI值明显降低(P < 0.05或P < 0.01)。病理切片结果显示,与空白组比较,模型组大鼠心肌细胞的细胞体积明显变大,且排列相对紊乱,同时细胞间距变宽,充斥着大量被蓝染的纤维。与模型组比较,针刺组大鼠心肌细胞体积更小,细胞间距更窄,细胞间隙蓝染的纤维变少。与空白组比较,模型组心肌组织的ACE2的表达水平和Ang(1-7)/AngⅡ显著降低(P < 0.01),ANP的表达水平升高(P < 0.01)。与模型组比较,针刺组ACE2的表达水平和Ang(1-7)/AngⅡ的比值显著升高(P < 0.01),ANP的表达水平降低(P < 0.01)。结论针刺“百会”“太冲”可通过调节ACE2/Ang(1-7)的表达,有效降低SHR血压,并减轻其心肌肥厚的程度。Objective To investigate the protective mechanism of acupuncture on myocardial hypertrophy by observing the changes of myocardial tissue structure and renin angiotension system (RAS) system action factor expression in spontaneously hypertensive rats (SHR). Methods Ten Wistar Kyoto rats (WKY) were selected as normal control group, 20 SHRs were divided into model group and acupuncture group by random number table method, with 10 rats in each group. All rats were SPF grade male 10-week-old rats. After one week of acclimatization, acupuncture was applied on Taichong (LR3) and Baihui (DU20) of SHRs in acupuncture group once a day for 28 days. The other two groups only received the same grasping and fixation stimulation. Blood pressure was measured before acupuncture on day 1, 7, 14, 21 and 28. At the end of the experiment, the weight of the whole heart and left ventricle of the rats were weighed, and ratio of left ventricle weight to body weight (LVWI), heart weight index (HWI) were calculated. HE and Masson staining method were applied to observe the myocardial tissue morphology, and Real-time RCR, Western blot, enzyme linked immunosorbent assay (ELISA) were used to detect the expression of atrial natriuretic peptide (ANP), angiotensin-converting enzyme 2 (ACE2), angiotensin (1-7)[Ang(1-7)], AngⅡ and Ang(1-7)/AngⅡ, as well as the underlying mechanisms were investigated. Results Compared with normal control group, the systolic blood pressure (SBP), HWI and LVWI in the model group were significantly increased (P < 0.01). Compared with the model group, SBP, HWI and LVWI values in the acupuncture group were significantly decreased (P < 0.05 or P < 0.01). The pathological results showed that compared with normal control group, the cell volume of rat cardiac muscle cells in the model group was significantly larger, and the arrangement was relatively disordered. Meanwhile, the cell spacing was widened, and there were a lot of blue-stained fibers. Compared with the model group, the cardiac muscle cells in the acupuncture

关 键 词：高血压 针刺 心肌肥厚 血管紧张素转换酶2 血管紧张素1-7 

分 类 号：R544.1[医药卫生—心血管疾病]