变棕溶杆菌OH23次生代谢产物抗菌活性的分析及其发酵培养基优化  

Study on the Antibacterial Activity of Secondary Metabolites from Lysobacter brunescens OH23 and Optimization of Fermentation Medium

作　　者：朱润杰 赵延存[2] 凌军 宋志伟[2] 赵杨扬[2] 薛雪 刘凤权[1,2] ZHU Runjie;ZHAO Yancun;LING Jun;SONG Zhiwei;ZHAO Yangyang;XUE Xue;LIU Fengquan(College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]南京农业大学植物保护学院,南京210095 [2]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国生物防治学报》2019年第3期426-436,共11页Chinese Journal of Biological Control

基　　金：江苏省农业科技自主创新资金(CX-16-1049);中国博士后面上项目(2017M610310)

摘　　要：本研究通过对变棕溶杆菌Lysobacter brunescens OH23菌株发酵产物的拮抗谱进行检测,发现其发酵产物对黄单胞属植物病原细菌具有特异拮抗活性,并且对水稻黄单胞菌不同菌株的拮抗活性存在差异。本研究进一步对OH23次生抗菌物质稳定性进行了分析,发现OH23产生的次生抗菌物质对高温、紫外光照、蛋白酶、非强碱性环境均表现稳定,但在强碱性环境(pH11.0)下拮抗活性丧失。最后,采用单因素试验和正交试验设计对OH23发酵培养基组分进行了优化,最终确定OH23高产次生抗菌物质的培养基配方为高聚蛋白胨5g/L、酵母粉5g/L、可溶性淀粉6g/L、FeSO4·7H2O15mg/L、ZnSO4·7H2O5mg/L。In this study,the antimicrobial spectrum of secondary metabolites of Lysobacter brunescens OH23was tested.The results showed that OH23 was a potential biocontrol strain with specific activity against Xanthomonas sp.At the same time,the secondary metabolites of OH23 showed different antagonistic activity against different strains of Xanthomonas oryzae.Stability test indicated that the active secondary metabolites were stable with high temperature,ultraviolet light,protease and non-strong alkaline environment.However,the antagonistic activity almost completely lost under strong alkaline conditions(pH=11.0).Single factor test and orthogonal design were used to screen and optimize the carbon source,nitrogen source and metal ion of OH23fermentation medium.The final formulation for high yield of active secondary metabolites included high polypeptone at 5 g/L,yeast powder at 5 g/L,soluble starch at 6 g/L,FeSO4·7H2O at 15 mg/L and ZnSO4·7H2O at5 mg/L.

关 键 词：变棕溶杆菌 水稻黄单胞菌 次生代谢产物 抗菌活性 培养基优化 

分 类 号：S476[农业科学—农业昆虫与害虫防治]