中国几省市猪萨佩罗病毒巢式RT-PCR检测与遗传进化分析  

Molecular Epidemiological Investigation of VP1 Gene of Porcine Sapelovirus in Several Provinces and Cities of China

作　　者：张备 李春秋[1] 苏明俊 刘秋瑾[1] 乔成鹏 朱金海[1] 孙东波[1] Zhang Bei;Li Chunqiu;Su Mingjun;Liu Qiujin;Qiao Chengpeng;Zhu Jinhai;Sun Dongbo(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agriculture University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学动物科技学院

出　　处：《黑龙江八一农垦大学学报》2019年第3期32-37,共6页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省杰出青年科学基金项目(JC2017007);黑龙江省博士后科研启动基金项目(LBH-Q16188);黑龙江省农垦总局科技攻关项目(HNK135-04-06-03)

摘　　要：为了准确了解猪萨佩罗病毒(Porcine Sapelovirus,PSV)在中国流行情况及遗传进化规律,试验针对PSV 5’-NTR基因利用巢式RT-PCR方法对2015~2017年来自中国部分省市规模化猪场采集的368份样品进行检测,将阳性样品对VP1基因进行克隆、测序、构建进化树。巢式RT^PCR检测结果表明,368份样品中24份样品为阳性,总阳性率为6.52%;8份样品测序成功,序列分析结果表明核苷酸同源性为77.0%~99.7%,推导的氨基酸序列同源性为83.6%~100.0%,遗传进化分析结果显示,中国地区的PSV病毒呈现出遗传多样性,其中3个毒株与德国参考毒株亲缘关系较近,2个毒株与国内外参考毒株的亲缘关系均较远,变异较大。研究丰富了PSV分子流行病学资料,为进一步研究该病毒提供了参考。In order to clarify the prevalence and genetic evolution of Porcine Saperovirus(PSV)n China,the nested RT-PCR method was used to test PSV 5’-NTR gene of 368 samples which were obtained from large-scale pig farm of several provinces and cities in China from 2015 to 2017.The VP1 of the positive samples were cloned,sequenced and constructed into a phylogenetic tree.Nested RT-PCR results showed that 24 samples were positive,and the total positive rate was 6.52%;8 samples were successfully sequenced,and the sequence analysis showed that the nucleotide homology was 77.0%-99.7%.The amino acid sequence homology was 83.6%-100.0%.The results of genetic evolution analysis showed that the PSV virus in China was genetic diversity.Three of the strains were closely related to the German reference strain,and other two strains had great difference and distant relative relationship from the reference strains in China and abroad.It enriched the epidemiological data of PSV molecules and provided a reference for further study of the virus.

关 键 词：猪萨佩罗病毒 VP1 基因 分子流行病学 

分 类 号：S852.651[农业科学—基础兽医学]