淹水胁迫对中山杉光合和荧光特性的影响  

Effects of waterlogging stress on photosynthesis and fluorescence of Taxodium hybrid

作　　者：江皓 张龙 张全全 饶辉 范俊俊 仲磊 张往祥 JIANG Hao;ZHANG Long;ZHANG Quanquan;RAO Hui;FAN Junjun;ZHONG Lei;ZHANG Wangxiang(College of Forestry,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;Forestry Bureau of Jiangsu Province,Nanjing,Jiangsu 210036,China;Yangzhou Small Apple Horticulture Co.,Ltd.,Yangzhou,Jiangsu 225200,China)

机构地区：[1]南京林业大学林学院,江苏南京210037 [2]江苏省林业局,江苏南京210036 [3]扬州小苹果园艺有限公司,江苏扬州225200

出　　处：《福建农林大学学报（自然科学版）》2019年第4期447-452,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：江苏省重点研发计划项目(BE2017375-2);国家科技支撑项目(2015BAD07B0104)

摘　　要：通过人工模拟淹水环境,对中山杉(Taxodium hybrid'zhangshan shan')光响应曲线、叶绿素快相荧光动力学曲线及其参数进行测定并分析.结果表明:(1)中山杉60d淹水后植株很少死亡,成活率为96.67%;(2)淹水胁迫下,中山杉叶片净光合速率(Pn)、光饱和点(LSP)和光补偿点(LCP)均显著低于对照(P<0.01),胞间CO2浓度(Ci)显著高于对照(P<0.01),而气孔导度(Gs)、表观量子效率(AQY)和蒸腾速率(Tr)差异不显著,但均低于对照;(3)叶绿素荧光动力学曲线均属于典型的OJIP曲线,但其荧光参数有显著差异.淹水的中山杉叶片在O相的初始荧光(Fo)高于对照,但在P相的最大荧光(Fm)显著低于对照(P<0.01),且淹水后在K相处的可变荧光Vk升幅(6%)大于J相处的可变荧光Vj(1.9%).淹水后,中山杉叶片单位面积的反应中心数量(RC/CSm)和PSⅡ电子受体库容量(Sm)变小,QA到QB过程中的电子传递受到抑制(ETo/CSm),PSⅡ向PSⅠ的电子传递能力参数中Ψo和φEo无显著差异,而φRo显著下降(P<0.01),最终表现为PSⅡ综合性能指数(PItotal)受到显著抑制(P<0.01).Photoresponse curve, chlorophyll fast phase fluorescence kinetic curve and its parameters of Taxodium hybrid were measured and analyzed under flooded and normal conditions. The results show that during the 60-day flooded condition, Taxodium hybrid rarely died, registering a survival rate of 96.67%. Net photosynthetic rate (Pn), light saturation point(LSP) and light compensation point (LCP) of T.hybrid were significantly lower under flooded condition than the control(P<0.01), while intercellular CO2 concentration (Ci) was significantly higher under flooded condition than the control(P<0.01). Overall, stomatal conductance (Gs ), apparent quantum efficiency (AQY) and transpiration rate (Tr) were lower under flooded condition, though no significant difference existed between both treatments. Chlorophyll fluorescence kinetic curves for two conditions both belonged to the typical OJIP curves, but whose fluorescence parameters were significantly different: under flooded condition, the initial fluorescence(Fo)in the O phase was significantly higher and the maximum fluorescence(Fm)in the P phase was significantly lower than the control(P<0.01);and a 6% increase of variable fluorescence Vk in the K phase after flooding was greater than the variable fluorescence Vj(1.9 %) in the J phase. After flooding treatment, the number of reaction centers per unit area(RC/ CSm )of leaves and the capacity of PSⅡ electron acceptor pool (Sm) decreased, and electron transfer from QA to QB was inhibited(ETo/ CSm). There was no significant difference between Ψo and φEo in parameters regarding electron transfer capability from PSⅡ to PSⅠ, while φRo decreased significantly (P<0.01), indicating that the PSⅡ comprehensive performance index (PItotal) was significantly inhibited(P<0.01).

关 键 词：淹水胁迫 中山杉 光合 荧光特性 

分 类 号：S728.6[农业科学—林木遗传育种]