PLLA/PCL复合纳米纤维三维多孔支架的制备及细胞相容性研究  

Preparation of Three-Dimensional Porous Scaffold Based on Poly(L-lactide acid)/ Polycaprolactone Composites Nanofibers and Cellular Biocompatibility

作　　者：林皓 刘瑞来 齐小宝 胡家朋 赵瑨云 付兴平 LIN Hao;LIU Rui-lai;QI Xiao-bao;HU Jia-peng;ZHAO Jin-yun;FU Xing-ping(Fujian Provincial Key laboratory of Eco-Industrial Green Technology,College of Ecological and Resources Engineering,Wuyi University.Wuyishan 354300,China)

机构地区：[1]福建省生态产业绿色技术重点实验室武夷学院生态与资源工程学院

出　　处：《高分子通报》2019年第6期60-67,共8页Polymer Bulletin

基　　金：国家自然科学基金(51406141);福建省自然科学基金(2018J05092,2019J01829,2019J01830);福建省教育厅项目(JT180550,JT180552,JT180553);南平市科技局(N2017DG03);福建省高校杰青项目(闽教科[2018]47号);福建省新世纪人才(闽教科[2018]47号)

摘　　要：以四氢呋喃(THF)为共溶剂,在无其它添加剂条件下,通过低温淬火、萃取、洗涤和干燥制得了PLLA/PCL H维纳米纤维多孔支架。利用扫描电镜.探讨PLLA/PCL比例、聚合物浓度和淬火温度等条件对纤维支架形貌影响。通过压缩强度、冲击强度、孔隙率、比表面积和体外生物活性等测试,考察PCL添加对支架机械强度和生物活性影响。实验结果表明,纤维是由PLLA结晶所形成,PCL的加入影响了 PLLA结晶,但提高了纤维支架的机械强度。最佳的实验条件为:PLLA/PVP = 8:2、聚合物浓度7 %(wt)、淬火温度一10℃。制得的三维纳米纤维多孔支架均一、规整,直径为150~500 nm,支架的压缩强度和冲击强度分别为17. 3 MPa和5. 6 kj/m^2、孔隙率和比表面积分別为96.3%和10.84 m^2/g。这样的孔隙率和比表面积为细胞的粘附、迁移、分化、增殖以及养料的传输提供了很好的条件.Poly( L-lactide acid)/polycaprolactone (PLLA/PCL) composites nanofibers three-dimensional porous scaffold was prepared by low-temperature quenching, extraction, washing and drying from tetrahydrofuran solution without the assistance of other additives. The effects of PLLA/PCL ratio, concentration and quenching temperature etc. on the morphology of fibrous scaffold was investigated by SEM. The influence of addition of PVP in mechanical property and bioactivity were also investigated through compression strength,impact strength. porosity, specific surface areas and in vitro bioactivity test. The results show that fibrous scaffold was produced by crystallization of PLLA. The addition of PCL impeded the crystallization of PLLA and increased mechanical strength of fibrous scaffold. The optimum test conditions as followed: PLLA/PCL = 8 : 2, concentration 7 %( wt), and juenching temperature 10℃,the uniform composites nanofibers there-dimensional porous scaffold with diameter of 150~500 nm was obtained. The compression strength and impact strength of fibrous scaffold were 17. 3 MPa and 5. 6 kJ/m2 , respectively. The porosity and specific surface areas of fibrous scaffold were 96. 3 % and 10. 84 mL g respectively. High porosity and specific surface areas will offer good condition of cell adhesion, cell differentiation, cell prolifer and nutrients transport.

关 键 词：聚乳酸 聚己内酯 纳米纤维 支架 多孔 

分 类 号：R318.08[医药卫生—生物医学工程] TQ342.94[医药卫生—基础医学]