抗菌素对杀白细胞素基因阳性耐甲氧西林金黄色葡萄球菌感染兔的疗效  

Therapeutic efficiency of antibiotics in rabbits infected with Panton-Valentine leukocidin-positive MRSA

作　　者：朱玉林[1] 李惠春 丁圣刚[1] ZHU Yulin;LI Huichun;DING Shenggang(Department of Pediatrics, First Affiliated Hospital, Anhui Medical University, Hefei 230022, CHINA)

机构地区：[1]安徽医科大学第一附属医院儿科

出　　处：《江苏医药》2019年第6期547-551,共5页Jiangsu Medical Journal

基　　金：安徽省自然科学基金(1408085QH157)

摘　　要：目的探讨两种抗菌素对杀白细胞素基因阳性[PVL(+)]耐甲氧西林金黄色葡萄球菌(MRSA)感染的治疗效应。方法在兔背部埋入一个中空、多孔的高尔夫练习球建立兔组织笼模型后,将16只新西兰雌兔随机均分为A、B、C和D四组,分别予以注射PVL(+)MRSA、PVL(-)MRSA、ATCC43300和ATCC25923菌株。当组织笼内细菌浓度达到1×10~8 CFU/ml时,每组随机选2只兔子予万古霉素治疗(A1、B1、C1、D1组),另2只予利奈唑胺治疗(A2、B2、C2、D2组)。每只兔子治疗前、治疗期间每次给药前及治疗达药物浓度稳态后1~3 d测定组织笼内细菌浓度。结果治疗前、后,A2组细菌浓度低于A1组(F=10560.216,P<0.01),B2组细菌浓度低于B1组(F=29.105,P<0.05),且随治疗时间的延长,A2组细菌浓度下降的幅度大于A1组(F=2052.739,P<0.01),B2组细菌浓度下降的幅度大于B1组(F=31.743,P<0.05)。而C1组与C2组(F=1.840,P>0.05)、D1组与D2组(F=1.468,P>0.05)间细菌浓度差异无统计学意义。结论利奈唑胺抗MRSA作用优于万古霉素,对PVL(+)MRSA导致的皮下软组织感染效果更明显。Objective To explore the therapeutic efficiency of two antibiotics in rabbits infected with Panton-Valentine leukocidin-positive [PVL(+)] methicillin-resistant Staphylococcus aureus(MRSA).Methods After established the rabbit tissue cage model,16 New Zealand female rabbits were equally randomized into four groups,which were respectively injected with PVL(+)MRSA(group A),PVL(-)MRSA(group B),ATCC43300(group C) and ATCC25923(group D).When the concentration of bacteria in the tissue cage reached to a level of 1×10~8 CFU/ml,2 rabbits in each group were treated with vancomycin(groups of A1,B1,C1 and D1) and other 2 rabbits were treated with linezolid(groups of A2,B2,C2 and D2).The bacterial concentration in each rabbit tissue cage was determined before treatment,during treatment and within 3 days after drug concentration homeostasis.Results The bacterial concentration in group A2 was lower than that in group A1(F=10560.216,P<0.01),while that in group B2 was lower than that in group B1(F=29.105,P<0.05) during treatment.With the prolongation of treatment time,the decrease of bacterial concentration in group A2 was greater than that in group A1(F=2052.739,P<0.01), and that in group B2 was greater than that in group B1(F=31.743,P<0.05).There was no significant difference in bacterial concentration between group C1 and group C2(F=1.840, P>0.05), so did between groups of D1 and D2(F=1.468, P>0.05).Conclusion Linazolamide has better anti-MRSA efficiency than vancomycin,especially in the subcutaneous soft tissue infection caused by PVL(+)MRSA.

关 键 词：利奈唑胺 万古霉素 耐甲氧西林金黄色葡萄球菌 杀白细胞素 兔组织笼模型 

分 类 号：R725[医药卫生—儿科]