水稻产量基因网络的初步研究  

Preliminary Studies on Rice Yield Gene Network

作　　者：王松文 程萌杰[1] 施利利 孙宁 张欣[1,2] 丁得亮 崔晶 边嘉宾[1,2] WANG Songwen;CHENG Mengjie;SHI Lili;SUN Ning;ZHANG Xin;DING Deliang;CUI Jing;BIAN Jiabin(College of Agronomy and Resource Environment,Tianjin Agricultural University,Tianjin 300384,China;China-Japan Joint Center on Palatability and Quality of Rice in Tianjin,Tianjin 300384,China)

机构地区：[1]天津农学院农学与资源环境学院,天津300384 [2]天津中日水稻品质食味研究中心,天津300384

出　　处：《海南师范大学学报（自然科学版）》2019年第2期163-169,共7页Journal of Hainan Normal University（Natural Science）

基　　金：国家水稻基因组计划重大专项(2017GK10102);天津市科技计划项目(16ZXZYNC00110)

摘　　要：产量是作物育种的首要目标。采用多个品种、多个对照品种对其蛋白质组学进行了解析,iTRAQ分析表明,水稻叶片和幼穗中有数万个功能蛋白表达,按照表达强度和产量的关联程度,鉴定了33个对产量有重要贡献的蛋白质,其中淀粉合成酶、种子储藏蛋白是产量构成的重要成分,采用酶活性、产量形成对糖苷合成酶及其基因调控网络进行了验证,对重要农艺性状基因克隆、基因网络重构等进行了讨论。Yield is the primary goal of crop breeding. Proteomics was analyzed using difference varieties and multiple con. trol varieties. iTRAQ analysis showed that there were tens of thousands of functional proteins expressed in rice leaves and young ears. According to the correlation between expression intensity and yield, 33 proteins were identified. Proteins with important contribution to yield, including starch synthases, seed storage proteins, and important components of these yields, were verified by enzyme activity and yield formation for glycosidic synthetase and its gene regulatory network. The cloning of important agronomic trait genes and the reconstruction of gene networks were discussed.

关 键 词：水稻 产量 蛋白质组 基因网络 

分 类 号：S336[农业科学—作物遗传育种]