长江流域4个野生大眼鳜群体的遗传多样性分析  

Genetic diversity of four wild Siniperca knerii populations in the Yangtze River

作　　者：程起群[1] 吕浩[1] 逄娇慧 赵金良[2] CHENG Qiqun;LYU Hao;PANG Jiaohui;ZHAO Jinliang(East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Oceanic andPolar Fisheries, Ministry of Agriculture and Rural Affairs, Shanghai 200090, China;Shanghai Ocean University, Laboratory of Freshwater Fisheries Germplasm Resources, Ministry of Agriculture andRural Affairs, Shanghai 201306, China)

机构地区：[1]中国水产科学研究院东海水产研究所,农业农村部远洋与极地渔业创新重点实验室,上海200090 [2]上海海洋大学,农业农村部淡水水产种质资源重点实验室,上海201306

出　　处：《中国水产科学》2019年第4期774-782,共9页Journal of Fishery Sciences of China

基　　金：现代农业产业技术体系专项资金项目(CARS-46)

摘　　要：为探讨长江流域大眼鳜(Siniperca kneri)野生群体的遗传多样性和遗传结构,为长江大眼鳜野生群体资源的有效管理和合理开发利用提供基础科学数据支撑,本研究基于线粒体细胞色素 b (cytochrome b, Cyt b)基因及控制区(D-loop)全序列,对 4 个群体(赤水河群体-CS、南京群体-NJ、岳阳群体-YY、宜昌群体-YC)共计 79 尾个体进行了分析。结果如下:(1)获得了长 1141 bp 的 Cyt b 基因全序列,共检测到 26 种单倍型。单倍型多样性(haplotype diversity,Hd)指数为 0.685 (CS)~0.924 (YC),核苷酸多样性(nucleotide diversity,π)指数为 0.176%(CS)~0.285%(YY)。群体内与群体间的遗传距离均在 0.002~0.003。(2)获得了长度为 834~840 bp 的 D-loop 全序列,在 79 尾个体中共检测到46 种单倍型。单倍型多样性指数为 0.754 (CS)~0.990 (NJ),核苷酸多样性指数为 0.548%(CS)~1.412%(YC)。群体内遗传距离在 0.005 (CS)~0.014 (YC),群体间遗传距离在 0.008~0.012,提示 4 个野生群体均尚未达到亚种分化水平。分子方差分析(AMOVA)显示群体内的变异是总变异的主要来源,长江流域大眼鳜野生群体无显著遗传结构差异。To investigate genetic diversity and structure of wild Siniperca knerii populations in the Yangtze River, a total of seventy-nine individuals from four wild populations (i.e., CS-Chishui River, NJ-Nanjing, YC-Yichang, and YY-Yueyang) were analyzed using the complete sequences of the mitochondrial markers Cyt b (cytochrome b) and D-loop (control region). The complete Cyt b sequences were 1141 bp in length, and 26 haplotypes were detected. The Cyt b haplotype diversity ranged from 0.685 (CS) to 0.924 (YC) and nucleotide diversity ranged from 0.176%(CS) to 0.285%(YY). Genetic distances within or between populations were both between 0.002 and 0.003. The whole D-loop sequences were 834–840 bp in length and 46 haplotypes were detected. D-loop haplotype diversity ranged from 0.754 (CS) to 0.990 (NJ) and nucleotide diversity ranged from 0.548%(CS) to 1.412%(YC). Genetic distances within populations were between 0.005 (CS) and 0.014 (YC), and from 0.008 to 0.012 among populations. The differences between these four populations of S. knerii have not risen to the subspecies level based on the genetic distances. Analysis of molecular variance (AMOVA) with Cyt b and D-loop sequences showed that most variance occurred within populations, suggesting that this is the main source of total variance.

关 键 词：长江 大眼鳜 细胞色素B基因 D-LOOP 遗传多样性 

分 类 号：S931[农业科学—渔业资源] Q9[农业科学—水产科学]