不同光源对闽西南黑母兔同期发情及激素分泌的影响  

Effect of Artificial Light Exposure on Estrus Synchronization and Hormone Secretion of Minxinan Black Female Rabbits

作　　者：孙世坤[1] 桑雷[1] 王锦祥[1] 陈冬金[1] 陈岩峰[1] 谢喜平[1] SUN Shi-kun;SANG Lei;WANG Jin-xiang;CHEN Dong-jin;CHEN Yan-feng;XIE Xi-ping(Institute of Animal Husbandry & Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院畜牧兽医研究所

出　　处：《福建农业学报》2019年第4期422-426,共5页Fujian Journal of Agricultural Sciences

基　　金：国家兔产业技术体系建设专项福州综合试验站任务(CARS-43-G-5);中央引导项目(2018L3004);福建省科技计划项目——省属公益类研究院所基本科研专项(2016R1022-12、2018R1023-14)

摘　　要：【目的】研究不同光源诱导闽西南黑母兔同期发情与激素之间的关系,以提高闽西南黑兔的繁殖性能。【方法】选用150日龄未经产雌性闽西南黑兔,于LED灯和节能灯下饲养1周,光照周期L∶D=16∶8h,对照组只提供自然光照。于补光前1d、光照3d后和光照7d后观察记录发情率并耳缘静脉采血。采用ELISA方法,研究不同光源对闽西南黑兔褪黑激素、促卵泡素和雌二醇分泌的影响。【结果】(1)LED灯和节能灯光组的发情率明显高于对照组,差异显著,表明LED灯和节能灯补光对闽西南黑母兔的发情率有明显影响;(2)ELISA试剂盒检测不同光源下血清中褪黑激素、促卵泡素和雌二醇素含量,LED灯和节能灯光处理使150日龄闽西南黑母兔在光照后3d和光照后7d血清中褪黑激素水平显著降低,而促卵泡素和雌二醇激素水平有增加的趋势,但差异不显著。【结论】不同光源长光照可以显著影响150日龄未经产雌性闽西南黑兔同期发情,且对血浆中的褪黑激素具有明显下降作用,但对促卵泡素和雌二醇影响较小。【Objective】 Effects of exposure to artificial lights on estrus synchronization and hormone secretion in female rabbits were investigated in order to improve the reproductive performance of Minxinan black rabbit.【Method】 Non-breeding Minxinan black female rabbits at the age of 150 d were raised under LED and energy-saving lamps or natural light for one week. A daily exposure cycle of L∶D=16∶8 h on the treatment animals or of the natural light conditions for the control group was applied. Estrus occurrence rates of the rabbits on both groups were recorded 1 d before as well as 3 d and 7 d after the beginning of the treatment. At the same time,blood samples were collected from the ear vein of the rabbits for serum melatonin,follicle stimulating hormone,and estradiol determinations by ELISA.【Result】(1) The artificial light exposure significantly increased estrus in the rabbits.(2) After 3 d and 7 d of the treatment,the serum melatonin in the rabbits decreased significantly,while the follicle stimulating hormone and estradiol increased slightly,whereas,no significant differences on those were detected in the control group.【Conclusion】The artificial light exposure appeared to significantly affect the estrus of 150-d-old non-parous Minxinan black female rabbits. It also exerted a significant effect on the serum melatonin,but not on the follicle stimulating hormone or estradiol in the rabbits.

关 键 词：LED 节能灯 同期发情 激素 闽西南黑兔 

分 类 号：S829.1[农业科学—畜牧学]