血管紧张素原基因M235T位点多态性与重度子痫前期的关系  

Relationship between M235T polymorphism of angiotensinogen gene and severe preeclampsia

作　　者：左金玲[1] 栾青春 姜海英[1] 李静[1] 崔春柳 ZUO Jinling;LUAN Qingchun;JIANG Haiying;LI Jing;CUI Chunliu(Tangshan Maternity and Children′s Hospital,Tangshan 063000,China)

机构地区：[1]唐山市妇幼保健院,河北唐山063000 [2]唐山弘慈医院

出　　处：《山东医药》2019年第19期31-34,共4页Shandong Medical Journal

基　　金：河北省医学科学研究课题计划(20191551)

摘　　要：目的探讨血管紧张素原(AGT)基因M235T位点多态性与重度子痫前期的关系。方法选择择期行剖宫产的重度子痫前期孕妇133例(观察组)、无指征剖宫产的正常孕妇93例(对照组),术日清晨采集无宫缩状态下外周静脉血3mL,采用聚合酶链反应-限制性片段长度多态性技术检测AGT基因M235T位点基因型和等位基因分布。结果两组AGT基因M235T位点基因型和等位基因分布比较差异均有统计学意义(χ^2分别为6.219、5.461,P均<0.05)。两组在校正高血压史这一混杂因素后,MT基因型者罹患重度子痫前期的危险性是MM基因型者的2.284倍(校正OR=2.284,95%CI:1.169~4.461,P<0.05);TT基因型者罹患重度子痫前期的危险性是MM基因型者的3.910倍(校正OR=3.910,95%CI:1.532~9.982,P<0.01)。两组MM、MT、TT基因型理论值与实际值比较P均>0.05,符合Hardy-Weinberg遗传平衡定律。结论AGT基因M235T位点多态性与重度子痫前期的发病有关,尤其是TT基因型。ObjectiveTo explore the relationship between angiotensinogen (AGT) M235T gene polymorphism and severe preeclampsia. Methods We selected 133 pregnant women with severe preeclampsia (observation group) who would undergo elective cesarean section and 93 normal pregnant women undergoing cesarean section (control group), and collected peripheral venous blood (3 mL) on the morning of operation day without uterine contraction. The genotype and allele distribution of M235T locus of AGT gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There were significant differences in genotype and allele distribution of M235T locus of AGT gene between the two groups (χ^2 =6.219, 5.461, respectively;both P<0.05). The risk of severe preeclampsia in patients with MT genotype was 2.284 times higher than that of patients with MM genotype (corrected OR =2.284, 95% CI : 1.169-4.461, P<0.05), and the risk of TT genotype was 3.910 times higher than that of MM genotype (corrected OR =3.910, 95% CI: 1.532-9.982, P<0.01). Comparisons of theoretical and actual values of MM, MT and TT genotypes between the two groups were all more than 0.05, which was in accordance with Hardy-Weinberg equilibrium law. Conclusion The M235T polymorphism of AGT gene is associated with the onset of severe preeclampsia, especially TT genotype.

关 键 词：重度子痫前期 血管紧张素原 单核苷酸多态性 

分 类 号：R714.24[医药卫生—妇产科学]