醒脑静注射液对脑出血大鼠神经功能以及脑血肿周围组织中内源性抗氧化系统Nrf2通路的影响  

Effect of Xingnaojing injection on neurological function of rats with cerebral hemorrhage and its effect on endogenous antioxidant system Nrf2 pathway in perihematomal tissue

作　　者：雷丹[1] 刘诸敏 卢武[1] 刘超轶 杨国平[2] LEI Dan;LIU Zhu-min;LU Wu(Department of Neurosurgery,Hanyang Hospital,Wuhan University of Science and Technology,Wuhan Hubei 430000,China)

机构地区：[1]武汉科技大学附属汉阳医院神经外科,湖北武汉430000 [2]武汉市第一医院神经外科,湖北武汉430000

出　　处：《临床和实验医学杂志》2019年第13期1354-1359,共6页Journal of Clinical and Experimental Medicine

基　　金：湖北省教育厅科学研究计划项目(编号:HU201735789)

摘　　要：目的分析醒脑静注射液对脑出血(ICH)大鼠神经功能以及脑血肿周围组织中内源性抗氧化系统Nrf2通路的影响。方法选取SPF级Wistar雄性大鼠75只,分为假手术组、模型组、醒脑静低、中、高剂量组,每组15只。除假手术组外,其他大鼠制备ICH模型,术后醒脑静低、中、高剂量组大鼠静脉注入醒脑静溶液,假手术组和模型组大鼠静脉注入生理盐水。观察各组大鼠神经功能缺损评分、脑含水量、脑组织病理形态、脑组织内氧化应激指标含量、核因子相关因子2(Nrf2)、醌氧化还原酶1(NQO1)及血红素加氧酶-1(HO-1)mRNA相对表达量及HO-1、Nrf2及Kelch样环氧氯丙烷相关蛋白-1(Keap1)蛋白表达。结果与假手术组相比,模型组大鼠脑组织Nrf2、NQO1及HO-1mRNA相对表达量上升;和模型组相比,醒脑静中、高剂量组大鼠脑组织Nrf2、NQO1及HO-1mRNA相对表达量上升,差异均有统计学意义(P<0.05),醒脑静低剂量组大鼠脑组织Nrf2、NQO1及HO-1mRNA相对表达量略微上升,差异无统计学意义(P>0.05)。结论醒脑静注射液可改善ICH大鼠神经功能,调节Nrf2-ARE通路及下游基因表达来降低机体氧化应激反应。Objective To analyze the effects of Xingnaojing injection on neurological function in rats with cerebral hemorrhage (ICH) and its effect on Nrf2 pathway in endogenous antioxidant system in cerebral hematoma. Methods Seventy-five SPF-level Wistar male rats were divided into sham operation group,model group,Xingnaojing low,medium and high dose groups,every group 15 rats. Except for sham operation group,other rats were prepared as ICH model. Rats in the middle and high dose groups were injected with Xingnaojing solution intravenously,and the rats in the sham operation group and the model group were injected with physiological saline intravenously. The neurological deficit score,brain water content,brain histopathology,brain tissue oxidative stress index,nuclear factor related factor 2 (Nrf2),quinone oxidoreductase 1 (NQO1) and heme oxygenation were observed. Relative expression of enzyme-1 (HO-1) mRNA and expression of HO-1,Nrf2 and Kelch-like epichlorohydrin-related protein-1 (Keap1) protein. Results Compared with the sham operation group,the relative expression of Nrf2,NQO1 and HO-1 mRNA in the brain tissue of the model group increased. Compared with the model group,the brain tissue Nrf2,NQO1 and HO- in the middle and high dose groups of the brain. The relative expression of mRNA increased,the difference was statistically significant ( P <0.05). The relative expression of Nrf2,NQO1 and HO-1 mRNA in the brain of Xingnaojing low dose group increased slightly,the difference was not statistically significant ( P >0.05). Conclusion Xingnaojing injection can improve the neurological function of ICH rats,regulate the Nrf2-ARE pathway and downstream gene expression to reduce the body's oxidative stress response.

关 键 词：大鼠 脑出血 继发性损伤 醒脑静注射液 神经功能 核因子相关因子2 

分 类 号：R285.5[医药卫生—中药学]