间充质干细胞条件培养液修复多种疾病损伤的潜能  

Conditioned medium from cultured mesenchymal stem cells: a potential for repairing multiple diseases and injuries

作　　者：邱纪玲 王晓彤 周灏雯 杨丕山 宋爱梅[1,2] Qiu Jiling;Wang Xiaotong;Zhou Haowen;Yang Pishan;Song Aimei(Shandong Provincial Key Laboratory of Oral Tissue Regeneration,Jinan 250100,Shandong Province,China;School of Stomatology,Shandong University,Jinan 250012,Shandong Province,China)

机构地区：[1]山东省口腔组织再生重点实验室,山东省济南市250100 [2]山东大学口腔医学院,山东省济南市250012

出　　处：《中国组织工程研究》2019年第29期4743-4748,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81771076),项目负责人:杨丕山~~

摘　　要：背景:间充质干细胞为再生医学的发展和多种疾病的治疗提供了新思路、新方法,但由于干细胞在受植床的移植率和成活率均较低,其作用机制更多倾向于旁分泌效应。条件培养液含有干细胞分泌的多种活性物质,具有易于获取、使用方便、安全性高等优点,将有可能作为一种新的技术用于人类多种疾病的治疗。目的:对间充质干细胞条件培养液的成分、功能、获取、应用等方面的进展做一综述,以期更好地应用于疾病治疗。方法:在PubMed、CNKI、万方数据库中,英文检索词为“Mesenchymal Stem Cells AND Conditionedmedium”,中文检索词为“间充质干细胞AND条件培养液,间充质干细胞AND条件培养基”,进行文献检索并筛选,最终纳入46篇进行综述。结果与结论:间充质干细胞条件培养液中含有间充质干细胞在生长过程中分泌的生长因子、细胞因子、炎性因子、细胞外基质蛋白和组织重塑酶等多种活性物质,具有趋化性能、免疫调节、促血管生成、细胞生长支持、保持干细胞活性或促进其他细胞转化为干细胞等功能。收集间充质干细胞生长过程中的培养液,经过过滤或浓缩等处理,可以获得不含细胞成分但含有更高浓度生长因子和细胞因子的活性液体,使用方便安全,现已将其用于基础研究、多种动物疾病模型和某些人体临床研究,并获得了较好的效果,随着对干细胞条件培养液的研究深入和不断优化,干细胞条件培养液的优势将会得到发挥,从而更好地造福于人类。BACKGROUND:Mesenchymal stem cells have provided new ideas and new methods for the development of regenerative medicine and the treatment of a variety of diseases.However,due to the low transplantation rate and survival rate of stem cells in the implanted bed,the mechanism of action of stem cells is inclined to involve the paracrine effect of soluble molecules released by stem cells during wound healing.The conditioned medium contains a variety of active substances secreted by stem cells.It is characterized by being easy to obtain,easy to use and safe,and may be used as a new technology for the treatment of a variety of human diseases.OBJECTIVE:To review the composition,function,acquisition and application of conditioned medium from mesenchymal stem cells,attempting to better apply it in the treatment of diseases.METHODS:The keywords were “mesenchymal stem cells AND conditioned medium” in English and Chinese,respectively.Relevant articles published were retrieved by the first author in the PubMed,CNKI,and WanFang.According to the inclusion and exclusion criteria,finally 46 articles were retained.RESULTS AND CONCLUSION:Conditioned medium obtained from mesenchymal stem cells contains growth factors,cytokines,inflammatory factors,extracellular matrix proteins,and tissue remodeling enzymes that are secreted by mesenchymal stem cells during the growth.The conditioned medium has the functions of immunomodulation,promotes angiogenesis,maintains stem cell activity,supports the growth of cells,has chemoattraction properties,and promotes cell differentiation into stem cells.The culture medium from cultured mesenchymal stem cells can be filtrated or concentrated to be an active liquid containing no cell components but higher concentrations of growth factors and cytokines,which is convenient and safe to use.This medium has been used for the basic research,a variety of animal disease models and some human clinical studies,and has achieved good results.With the in-depth research the stem cell conditioned medium will be cont

关 键 词：间充质干细胞 条件培养液 旁分泌 细胞因子 生长因子 再生医学 国家自然科学基金 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]