茶溴香酰胺脂质体对黑色素瘤细胞生长和迁移的抑制作用  

Inhibitory Effect of TBrC-L on Melanoma Cell Growth and Migration

作　　者：张欣 张伟伟 张燕 石晓玉 孙紫薇 刘昆[1] 张国营[1] ZHANG Xin;ZHANG Wei-wei;ZHANG Yan;SHI Xiao-yu;SUN Zi-wei;LIU Kun;ZHANG Guo-ying(Laboratory of Molecular Pharmacology, School of Pharmacy, Yantai University, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, China)

机构地区：[1]烟台大学药学院分子药理学实验室烟台大学新型制剂与生物技术药物研究山东省高校协同创新中心分子药理和药物评价教育部重点实验室(烟台大学)

出　　处：《烟台大学学报（自然科学与工程版）》2019年第3期262-270,共9页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金资助项目(ZR2015HM004)

摘　　要：考察茶溴香酰胺脂质体(TBrC-L)对黑色素瘤B16细胞生长和迁移的抑制作用及其分子机制,为研发抗癌新药提供科学依据。采用MTT法和细胞划痕术探究TBrC-L对黑色素瘤B16细胞生长和迁移的影响;Hoechst33258染色法观察药物作用下细胞凋亡形态的变化;WesternBlotting检测TBrC-L对B16细胞生长、迁移和凋亡相关蛋白表达的影响。结果表明:TBrC-L能对B16细胞的生长和迁移产生显著性抑制作用,并且诱导其凋亡;TBrC-L可下调c-Met、HGF、Bcl-2、NF-κB和VEGFR2的表达,上调Caspase-3、E-cadherin、Bax、p53和Cytochromec的表达,抑制HGF/Met/VEGFR2-NF-κB通路可能是TBrC-L抑制黑色素瘤B16细胞生长和迁移作用的重要分子机制。本研究结果提示,TBrC-L具有进一步开发为抗黑色素瘤药物的潜力。The purpose of this study is to investigate the inhibitory effect of theanine derivative TBrC-prepared liposomes (TBrC-L) on the growth and migration of melanoma B16 cells. The effects of different concentrations of TBrC-L on the growth and migration of melanoma B16 cells are investigated by using MTT and cell scratch tests respectively. Hoechst 33258 staining is used to observe the change of cell morphology under the action of drugs. Western blotting is used to analyze the related protein expressions in the melanoma cells. TBrC-L significantly inhibits the growth and migration of the melanoma B16 cells and induces the apoptosis in the melanoma cells. TBrC-L significantly reduces the protein expressions of c-Met, HGF, Bcl-2, NF-κB and VEGFR2, while it remarkably enhances the protein expressions of caspase-3, E-cadherin, Bax, PARP, p53 and cytochrome c in B16 cells. The molecular mechanism of TBrC-L action may be associated with the inhibition of HGF/Met/VEGFR2-NF-κB signaling pathway. The results of this study indicate that TBrC-L has the potential for further development of anti-melanoma drugs.

关 键 词：茶溴香酰胺脂质体 黑色素瘤 生长和迁移 分子机制 

分 类 号：TG376.2[金属学及工艺—金属压力加工]