基于微流控芯片荧光检测的LED光源自适应补偿方法  

Adaptive Compensation Method for LED Light Source Based on Fluorescence Detection of Microfluidic Chips

作　　者：樊夏辉 刘桂礼[1] 孔全存[1,2] 李勇 王志强 钟昊[2,3] 徐涛 FAN Xia-hui;LIU Gui-li;KONG Quan-cun;LI Yong;WANG Zhi-qiang;ZHONG Hao;XU Tao(School of Instrumentation Science and Opto-Electronics Engineering,Beijing Information Science and Technology University,Beijing 100192,China;Tianjin Research Institute for Advanced Equipment,Tsinghua University,Tianjin 300300,China;Department of Mechanical Engineer,Beijing Key Laboratory of Precision/Ultra-precision Manufacturing Equipment and Control,Tsinghua University,Beijing 100084,China)

机构地区：[1]北京信息科技大学仪器科学与光电工程学院,北京100192 [2]清华大学天津高端装备研究院,天津300300 [3]清华大学机械工程系精密/超精密制造装备及控制北京市重点实验室,北京100084

出　　处：《仪表技术与传感器》2019年第6期118-122,共5页Instrument Technique and Sensor

基　　金：国家自然科学基金项目(51675054);北京市自然科学基金项目(3172013);天津市自然科学基金项目(17JCZDJC32300);北京市教委科研计划项目(KM201711232005)

摘　　要：针对微流控芯片免疫荧光检测中LED光源不稳定导致检测区和质检区荧光采集同步实时性较差的问题,提出了一种基于光源漂移特性的自适应补偿方法。通过分析光源呈负指数形式衰减的漂移特性,引入光源时变补偿因子,对光源进行自适应补偿,实现微流控芯片高精度、高稳定性检测。对比实验结果表明光源时变补偿因子具有自适应性,且经过补偿后,免疫荧光检测的分辨率达到0.01ng/mL,CV值(离散系数,coefficient of variation)提高约50%,验证了其应用可行性。For solving the problem of the poor synchronous real time of the fluorescence acquisition between the detection ar ea and the quality control area caused by LED(light emitting diode) light source unsteadiness in immunofluorescence staining of microfluidic chips,an adaptive compensation method based on the drift characteristics of light source was put forward.The time varying compensation factor for light source was obtained by analyzing the drift characteristics of the attenuation of the light source in a negative exponential form.And the adaptive compensation for light source was carried out to finish high precision and high stability detection for microfluidic chips.The contrast experimental results show that the time varying compensation factor for light source has adaptability. The resolution of immunofluorescence staining reached 0. 01 ng/ mL and the value of coefficient of variation increased by 50% after adaptive compensation,and its application feasibility was validated.

关 键 词：微流控芯片 免疫荧光检测 漂移特性 自适应补偿 CV值 

分 类 号：TN247[电子电信—物理电子学]