聚丙烯酰胺降解组合菌的筛选和降解条件优化  

Screening and degradation conditions of degrading bacteria with polyacrylamide

作　　者：任国领 李蔚[3] 施寒清[4] 卞立红 戚桂娜 胡敏 REN Guoling;LI Wei;SHI Hanqing;BIAN Lihong;QI Guina;HU Min(Heilongjiang Provincial Key Laboratory of Oilfield Applied Chemistry and Technology,Daqing,Heilongjiang 163712,China;School of Biotechnology,Daqing Normal University,Daqing,Heilongjiang 163712,China;Exploration and Development Research Institute,Daqing Oilfield Company Limited,Daqing,Heilongjiang 163712,China;School of Petroleum Engineering,Northeast Petroleum University,Daqing,Heilongjiang 163318,China;Daqing Huali Biological Technology Company Limited,Daqing,Heilongjiang 163414,China)

机构地区：[1]黑龙江省油田应用化学与技术重点实验室,黑龙江大庆163712 [2]大庆师范学院生物工程学院,黑龙江大庆163712 [3]大庆油田有限责任公司勘探开发研究院,黑龙江大庆163712 [4]东北石油大学石油工程学院,黑龙江大庆163318 [5]大庆华理生物技术有限公司,黑龙江大庆163414

出　　处：《东北石油大学学报》2019年第3期109-114,I0007-I0008,共8页Journal of Northeast Petroleum University

基　　金：黑龙江省青年创新人才计划项目(UNPYSCT-2015116);大庆市科技局项目(zd-2017-43)

摘　　要：从大庆油田含聚污水中分离得到3株聚丙烯酰胺降解菌(N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1),进行生理生化检测和16S rDNA分子生物学鉴定,在不同pH条件下混合培养,研究3株聚丙烯酰胺降解菌的最优降解组合、降解pH和降解时间。结果表明:3株聚丙烯酰胺降解菌分别属于纤维微菌属、不动杆菌属和假单胞菌属。在pH为7.0时,N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1组合的降解率最大,最优降解时间为7d时,降解率为39.5%;在pH为9.0时,N2-2-P139-D1和N2-2-P140-X1组合的降解率最大,最优降解时间为5d时,降解率为34.5%。复合菌N2-2-P139-D1和N2-2-P140-X1,以及复合菌N2-2-P139-D1、N2-2-P140-D1和N2-2-P140-X1对现场含聚污水中聚丙烯酰胺的最大降解率分别为35.4%和31.0%,最优降解时间为5d。该结果为微生物在大庆油田采出液油水分离和含聚污水处理提供支持。In this study,three HPAM-degrading bacterial strains named by N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1 were isolated from polymer-containing wastewater in Daqing Oilfield.The results of physiological and biochemical tests and analysis of 16S rDNA sequence showed that these strains belonged to Cellulosimicrobium sp.,Acinetobacter sp.and Pseudomonas sp.respectively.In order to study the degradation combination,pH value and degradation time of Polyacrylamide in polymer-bearing wastewater,the bacteria were mixed in different pH environments.The results showed that: the optimal combination was N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1,the degradation rate was 39.5% in pH 7.0,and the optimal time for degradation was 7 days;the optimal combination of N2-2-P139-D1 and N2-2-P140-X1,the degradation rate was 34.5% in pH 9.0,and the optimal time for degradation was 5 days.The polyacrylamide degradation rate of polymeric wastewater was 35.4% and 31.0% respectively by the combination of N2-2-P139-D1 and N2-2-P140-X1,and the combination N2-2-P139-D1,N2-2-P140-D1 and N2-2-P140-X1,and the optimal degradation time was 5 days.The results can provide some technical support for the application of microorganisms in oil-water separation and polymer-bearing wastewater treatment in Daqing Oilfield.

关 键 词：聚丙烯酰胺 复合菌 降解率 含聚污水 大庆油田 

分 类 号：TE992[石油与天然气工程—石油机械设备]