全氟化碳汽化吸入对原发肺冲击伤犬肺组织相关microRNA的影响  

The differentially expressed miRNA in lung tissue induced by vaporized perfluorocarbon inhalation (VPFCI) in primary blast lung injuried canines

作　　者：李怀东 张兆瑞[2] 陈良安[2] LI Huai-dong;ZHANG Zhao-rui;CHEN Liang-an(Tai'an Branch,No. 960 Hospital of PLA,Tai'an,Shandong 271000,China)

机构地区：[1]解放军第960医院泰安院区,山东泰安271000 [2]解放军总医院,北京100853

出　　处：《实用医药杂志》2019年第7期643-647,共5页Practical Journal of Medicine & Pharmacy

基　　金：军队十二五重点项目(BWS11J057)

摘　　要：目的探讨全氟化碳汽化吸入(VPFCI)对肺冲击伤犬肺组织的miRNA影响。方法健康杂种犬18只(雌雄不限),随机分为2组:单纯肺冲击伤组(B组):给予小型冲击波发生器制作肺冲击伤模型。肺冲击伤全氟化碳汽化吸入通气组(BMP组):给予小型冲击波发生器制作肺冲击伤模型后,常规机械通气基础上予全氟化碳汽化吸入。实验结束后留取肺脏组织,应用miRNA芯片筛选全氟化碳汽化吸入作用肺冲击伤犬肺组织差异表达的miRNA及其靶基因。结果miRNA芯片筛选全氟化碳作用于肺冲击伤犬肺组织差异表达的miRNA23条及其靶基因449个。结论全氟化碳汽化吸入可能通过影响肺冲击伤犬相关miRNA及其靶基因的表达发挥生物学作用。Objective To investigate the differentially expressed miRNA in lung tissue of primary blast lung injuried canines by VPFCI.Methods Eighteen males or females crossbreed canines were divided randomly into two groups:BLI group(B)and BLI+MV+PFC group(BMP).The lung tissues were harvested at the end of the experiment.Differentially expressed miRNAs between B group and BMP group were detected by high-throughput study of miRNA chip.Results Totalled 23 differentially expressed miRNA and Totalled 449 target genes between B group and BMP group were detected by high-throughput study of miRNA chip.Conclusion Differentially expressed miRNA show that VPFCI may be playing a role on BLI.

关 键 词：原发肺冲击伤 全氟化碳吸入(VPFCI) MICRORNA 

分 类 号：R826[医药卫生—临床医学]