小麦G6PDH基因的生物信息学分析及其低温胁迫下苗期的表达特征  

Bioinformatics Analysis of Wheat G6PDH Genes and Their Expression Patterns in Tillering Node and Leaf under Cold Stress

作　　者：田宇[1] 彭瞰看 宋春华 于萍[1] 刘楠[1] 包雨卓 孟婧[1] 徐庆华[1] 苍晶[1] TIAN Yu;PENG Kankan;SONG Chunhua;YU Ping;LIU Nan;BAO Yuzhuo;MENG Jing;XU Qinghua;CANG Jing(College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030,China)

机构地区：[1]东北农业大学生命科学学院

出　　处：《麦类作物学报》2019年第6期631-638,共8页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31471423);国家自然科学青年基金项目(31601236)

摘　　要：葡萄糖-6-磷酸脱氢酶(G6PDH)是戊糖磷酸途径(PPP)的关键酶,可为小麦(Triticum aestivum)的生物合成提供NADPH以及一些中间代谢产物,并且在小麦生长发育及响应胁迫方面也发挥重要作用。本研究对基因组数据库中获得的12条小麦G6PDH蛋白序列及其基因序列进行一系列生物信息学分析,结果表明,12条小麦G6PDH蛋白分别定位于细胞质及质体中,且均为亲水、不稳定蛋白。从系统发生树的进化和亲缘关系上可以看出,12条小麦G6PDH蛋白分别属于胞质和质体亚型。不同亚型的G6PDH蛋白保守Motif的种类和数量有所不同,不同亚型的基因结构也具有明显差别。qRT-PCR结果表明,低温胁迫下,3种亚型(胞质亚型、质体P1和P2亚型)的小麦TaG6PDH基因均被不同程度地诱导表达,并且均随着温度的降低而升高,预示着G6PDH在小麦抗寒方面发挥正向调节作用。Glucose-6-phosphate dehydrogenase(G6PDH)is a key enzyme in the pentose phosphate pathway.It provides NADPH and some intermediate metabolites for the biosynthesis of wheat(Triticum aestivum),and it also plays an important role in wheat growth and development and stress response. In this study,a series of bioinformatics analyses were performed on 12wheat G6PDH protein sequences and their gene sequences obtained from the genomic database.The results showed that 12 wheat G6PDH proteins are localized in cytoplasm and plastid,and they are all hydrophilic and unstable proteins.From the evolution and phylogenetic relationship of phylogenetic tree,it can be saw that 12 wheat G6PDH proteins belong to cytoplasmic and plastid subtypes.The types and numbers of conserved motifs of different subtypes of G6PDH proteins are also different.Gene structure analysis also demonstrated significant differences in the genetic structure of different subtypes.The results of qRTPCR showed that under the conditions of cold stress,the TaG6PDH genes of three subtypes(cytoplasmic subtype,plastid P1and P2subtypes)were induced to different degrees and showed an increasing trend with decreasing temperature.It is indicated that G6PDH plays a positive role in regulating wheat cold resistance.

关 键 词：小麦 TaG6PDH基因 逆境胁迫 表达模式 

分 类 号：S512.1[农业科学—作物学] S330