PDR患者视网膜前膜中miR-126和VEGF的表达及意义  

Expressions and Significances of MiR-126 and VEGF in the Anterior Retinal Membrane of patients with PDR

作　　者：金益[1] 卢珊珊[1] 牟晓月[2] Jin Yi;Lu Shanshan;Mou Xiaoyue(Department of Ophthalmology, Taizhou First People's Hospital, Zhejiang 318020, China)

机构地区：[1]台州市第一人民医院眼科,318020 [2]台州市第一人民医院风湿免疫科,318020

出　　处：《医学研究杂志》2019年第6期95-99,共5页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(LY15H130018)

摘　　要：目的研究miR-126和血管内皮生长因子(VEGF)在增殖性糖尿病视网膜病变(PDR)视网膜前膜中的表达及其与新生血管生成的关系.方法将2015年4月~2018年4月在笔者医院眼科行玻璃体切除术的PDR患者80例(80眼)作为研究对象(PDR组),同时期行玻璃体切除术的50例(50眼)特发性黄斑裂孔(IMH)患者作为对照(IMH组),分别采集患者视网膜前膜组织,RT-PCR检测前膜组织中miR-126、VEGF mRNA、CD34 mRNA表达水平,免疫组织化学染色检测VEGF和CD34在视网膜前膜中表达情况,并计数微血管密度,分析PDR患者视网膜前膜组织中miR-126表达水平与VEGF、CD34的相关性.结果 PDR组患者视网膜前膜组织中miR-126相对表达量显著低于IMH组患者(P<0.05),VEGF mRNA、CD34 mRNA相对表达量显著高于ROV组患者(P<0.05);免疫组化染色结果,PDR组患者视网膜前膜中VEGF、CD34均呈阳性表达,且多于IMH组患者,PDR组患者视网膜前膜中VEGF IA值、MVD均显著高于IMH组患者(P<0.05);PDR组患者视网膜前膜组织中miR-126与VEGF表达水平呈显著负相关(r=-0.543,P=0.014),与CD34表达呈显著负相关(r=-0.730,P=0.000),VEGF与CD34表达水平呈显著正相关(r =0.571,P=0.005).结论 PDR患者视网膜前膜中miR-126表达降低,VEGF表达升高,可能是PDR新生血管形成的重要调控机制.Objective To investigate the expressions of miR - 126 and vascular endothelial growth factor( VEGF) in the anterior retinal membrane of proliferative diabetic retinopathy( PDR) and their relationships with neovascularization. Methods Eighty cases( 80 eyes) of PDR patients who underwent vitrectomy in our hospital from April 2015 to April 2018 were selected as subjects( group PDR ). Fifty patients(50 eyes) with idiopathic macular hole( IMH ) who underwent vitrectomy at the same time were as controls( group IMH ). The tissues of the anterior retinal membrane were collected, respectively. RT - PCR was used to detected the expression of levels of miR - 126 , VEGF mRNA and CD34 mRNA in the anterior membrane tissue. Immunohistochemistry was used to detect the expressions of VEGF and CD34 in the epiretinal membrane, and the microvascular density was counted. The correlations between the expression of miR - 126 with VEGF and CD34 in the epiretinal membrane of PDR patients were analyzed. Results The relative expression of miR - 126 in group PDR was significantly lower than that in group IMH( P <0. 05 ). The relative expressions of VEGF mRNA and CD34 mRNA were significantly higher than those in groupROV ( P < 0. 05 ). Immunohistochemical staining showed that the expressions of VEGF and CD34 in the retina of group PDR were positive, and were more than the those of group IMH. The values of VEGF IA and MVD in anterior retinal membrane of group PDR were significantly higher than those of group IMH ( P < 0. 05 ). The miR - 126 expression level in the epiretinal membrane tissue of group PDR was significantly negatively correlated with the VEGF expression level( r =-0. 543 , P =0.014), was significantly negatively correlated with the CD34 expression level( r =- 0. 730, P = 0. 000). VEGF expression level was significantly positively correlated with the CD34 expression level( r = 0. 571 , P = 0. 005 ). Conclusion The expression of miR - 126 is decreased and the expression of VEGF is increased in the epiretinal membrane of PDR patien

关 键 词：增殖性糖尿病视网膜病变 MIR-126 血管内皮生长因子 CD34 视网膜前膜 

分 类 号：R587.2[医药卫生—内分泌]