梨ERF超家族全基因组鉴定及表达分析  

Genome-wide Identification and Their Expression Analysis of Ethylene-Responsive Factors (ERF) Superfamily in Pear

作　　者：钟必凤[1] 邓家林[1] 李文贵[1] 张全军[1] Zhong Bifeng;Deng Jialin;Li Wengui;Zhang Quanjun(Key Laboratory of Horticultural Crops, Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture, Horticultural Research Instituteof Sichuan Academy of Agricultural Sciences, Chengdu, 610066)

机构地区：[1]四川省农业科学院园艺研究所农业部西南地区园艺作物生物学及种质创制重点实验室

出　　处：《分子植物育种》2019年第12期3874-3886,共13页Molecular Plant Breeding

基　　金：四川省财政育种工程青年基金(2017QNJJ-008);成都市科技项目(2015-NY02-00009-NC)共同资助

摘　　要：中国南方地区普遍存在的由于干旱等原因提早落叶导致的梨二次花现象给生产带来了很大的损失,且该过程可以通过人工摘叶处理进行模拟诱导。乙烯响应因子ERF成员被认为参与了植物的激素应答、生物和非生物胁迫以及生长发育调控过程。本研究基于公开的白梨基因组序列,利用生物信息学方法对其中的ERF因子进行鉴定,并对其分组及各组结构域进行了详细分析。结合课题组采用摘叶诱导二次花形成过程中的梨转录组数据,分析了响应该过程的ERF成员。结果表明,共获得了 211个白梨ERF家族编码基因,并可根据系统进化关系和结构域保守性分为11个组,其中种特有组一个。砂梨摘叶诱导二次花过程的转录组数据分析表明,有37个ERF因子差异表达,摘叶初期(摘叶后7 d)无响应,中期(摘叶后17 d)以下调表达为主,后期上调。对其中的4个差异表达基因进行了实时荧光定量PCR分析验证,结果与转录组分析结果吻合。此研究进一步丰富了梨响应胁迫反应的ERF基因资源,为进一步探讨ERF在成花过程中的作用提供了理论依据。The returning blooming phenomenon in pears, which is commonly found in southern China due to drought or other reasons, has brought great losses in pear production. The process can be simulated and induced by manual defoliation(DF). Ethylene response factors(ERF) are thought to be involved in plant hormone response, biotic or abiotic stress, growth and development regulation processes. In this study, based on the published genome sequence of white pear, we used the bioinformatics to identify ERF factors. Phylogeny and conserved structural domains were analyzed in detail. The transcriptome data derived from Pyrus pyrifolia Nakai cv. Hosui bud release following early defoliation were used to investigate those involved ERF members. The results showed that a total of211 ERF encoding genes were obtained. They could be divided into 11 groups based on phylogenetic relationships and structural conservation, including one species specific group. The bioinformatics analysis of the transcriptomic data revealed that 37 ERF factors were differentially expressed compared with undefoliated control. In the beginning of defoliation(7 days after DF, DF7), there were no ERF member responses. During the middle stage(DF17), most responsive genes were down-regulated. Large proportion of the responsive genes was up-regulated in the last stage. Four differentially expressed genes were picked and verified by real-time quantitative PCR. The results were consistent with the results of transcriptomic analysis. Results of this study could further enrich the ERF gene resources for pear in stress response, and also provide a theoretical basis for further exploration of the role of ERFs in the flowering process.

关 键 词：梨 乙烯响应因子 二次花 基因家族 

分 类 号：S661.2[农业科学—果树学]