基于银耳转录组测序的多糖代谢途径分析  

Study on Fructose and Mannose Metabolism Pathway of Tremella fuciformis Based on Transcriptome

作　　者：王东[1] 牛蓓[2] 宋君[1] 赵树海 雷绍荣[1] 郭灵安[1] 张富丽[1] 刘文娟[1] 常丽娟[1] 赵黎明[4] WANG Dong;NIU Bei;SONG Jun;ZHAO Shu-hai;LEI Shao-rong;GUO Ling-an;ZHANG Fu-li;LIU Wen-juan;CHANG Li-juan;ZHAO Li-ming(Analysis and Testing Center,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China;College of Medicine,Chengdu University,Sichuan Chengdu 610106,China;Institute of Science and Technology Research for Tremella fuciformis in Tongjiang,Bazhong,Sichuan Tongjiang 636700,China;Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China)

机构地区：[1]四川省农业科学院分析测试中心,四川成都610066 [2]成都大学医学院,四川成都610106 [3]巴中市通江银耳科学技术研究所,四川通江636700 [4]四川省农业科学院,四川成都610066

出　　处：《西南农业学报》2019年第6期1347-1352,共6页Southwest China Journal of Agricultural Sciences

基　　金：四川省财政创新能力提升工程专项资金项目(2013G XJS-014)

摘　　要：【目的】对银耳进行转录组测序分析,分析多糖生物合成途径和挖掘关键功能基因。【方法】采用Illumina Hiseq 2500测序平台对银耳菌丝体进行测序,组装得到unigene序列,并与公共数据库进行对比分析和注释,挖掘果糖和甘露糖代谢通路相关基因。【结果】本研究共得到4.72 Gb raw data,拼接后获得17 008条unigene序列,N50为2073 bp;注释结果表明,76.47 %序列能够注释到Uniprot公共数据库,注释到GO分类和KEGG通路的unigene分别为10 152和5671条。进一步分析银耳果糖和甘露糖代谢途径相关基因,挖掘得到74条unigene;其中编码L-iditol 2-dehydrogenase的unigene最多,其次是butanol dehydrogenase。【结论】基于转录组测序数据解析及预测,为研究银耳多糖和其它产物的生物合成途径及分子机制提供了数据支持,也为银耳品质的形成提供理论依据。【Objective】The study aimed to study on the expression of genes involved in the biosynthesis of fructose and mannose metabolism and explore the functional genes in Tremella fuciformis ,the transcriptome was sequenced.【Methods】 By Illumina Hiseq 2500 platform sequencing technology,the transcriptome of the mycelium was sequenced,assembled and annotated. Moreover,unigene related to the fructose and mannose metabolism pathway were identified.【Results】4.27 Gb raw data was generated,and 17 008 unigenes were obtained after splicing,which the N50 was 2073 bp. 76.47 % unigene was annotated in the Uniprot database,which 10 152 and 5671 unigenes were annotated in the GO classification and KEGG pathways. The fructose and mannose metabolism pathway in Tremella fuciformis involved in 74 unigenes,especially unigene coding L-iditol 2-dehydrogenase and butanol dehydrogenase with the most unigenes.【Conclusion】This study would be established for revealing the biosynthesis mechanism of polysaccharide and other production. At the same time,this study will provide a theoretical basis for quality formation of Tremella fuciformis .

关 键 词：银耳 转录组 果糖和甘露糖代谢途径 

分 类 号：S646.6[农业科学—蔬菜学]