正交设计筛选通关藤增殖培养基的研究  

Study on Screening of Proliferation Medium of Marsdenia tenocissima by Orthogonal Design

作　　者：韩俊[1] 王建军[1] 龙光强[1] 沈雪梅[2] 余珊 HAN Jun;WANG Jian-jun;LONG Guang-qiang(Yunnan Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]云南农业大学,云南昆明650201 [2]昆明市农业科学研究院,云南昆明650034

出　　处：《安徽农业科学》2019年第13期163-165,170,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(81660636);云南省科技计划项目(2016RA009)

摘　　要：[目的]应用正交试验设计方法,筛选通关藤增殖培养基。[方法]以通关藤的诱导苗为试验材料,采用L9(34)正交试验设计方案研究培养基、6-BA(细胞分裂素)、NAA(萘乙酸)及不同接种部位4个试验因子对通关藤增殖苗生长状况和增殖率的影响。[结果]筛选出通关藤最佳增殖培养基方案为MS+0.5mg/L6-BA+0.3mg/LNAA+25g/L蔗糖+7g/L琼脂+茎尖。[结论]该研究为通关藤规模化生产提供一定的技术支持。[Objective]The orthogonal experiment design method was used to screen the proliferation medium of Marsdenia tenocissima .[Method]The induced seedlings of Marsdenia tenocissima were used as experimental materials, and the effects of four test factors of 6-BA (cytokinin), NAA (naphthaleneacetic acid) and different inoculation sites on the growth status and proliferation rate of proliferation seedlings of Marsdenia tenocissima were studied by L 9(3 4) orthogonal design.[Result]The optimal proliferation medium of Marsdenia tenocissima was MS+ 0.5 mg/L 6-BA+0.3 mg/L NAA+25 g/L sucrose+7 g/L agar+stem tip.[Conclusion]This research provides certain technical support for for scale production of Marsdenia tenocissima.

关 键 词：通关藤 增殖培养基 组织培养 激素 正交试验 

分 类 号：S567[农业科学—中草药栽培]