转化生长因子β1、Survivin和Caspase-3蛋白在肝内胆管结石合并胆管狭窄患者组织中的表达及意义  

Expressions and significance of transforming growth factor β1, Survivin and Caspase-3 in patients with intrahepatic bile duct stones and bile duct stenosis

作　　者：谢伟选 罗昆仑[2] 方征[2] 刘洪[2] 夏咸军[2] 周志华[3] 赵海滨[3] Xie Weixuan;Luo Kunlun;Fang Zheng;Liu Hong;Xia Xianjun;Zhou Zhihua;Zhao Haibin(Wuxi Clinical School of Anhui Medical University,Wuxi 214044,Jiangsu Province,China;Department of Hepatobiliary Surgery,The 904th Hospital of Joint Logistic Support Force of PLA,Wuxi 214044,Jiangsu Province,China;Department of Pathology,The 904th Hospital of Joint Logistic Support Force of PLA,Wuxi 214044,Jiangsu Province,China)

机构地区：[1]安徽医科大学无锡临床医学院,无锡214044 [2]中国人民解放军联勤保障部队第904医院肝胆外科,无锡214044 [3]中国人民解放军联勤保障部队第904医院病理科,无锡214044

出　　处：《中华肝胆外科杂志》2019年第6期452-456,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：南京军区医药卫生科研基金(14D05).

摘　　要：目的探讨肝内胆管结石合并胆管狭窄患者肝内胆管组织中转化生长因子(TGF)β1、Survivin和Caspase-3三种标志物的表达及意义。方法收集中国人民解放军联勤保障部队第904医院病理科2013年至2018年患者肝内胆管组织蜡块标本130例。应用免疫组化法检测50例肝内胆管结石合并胆管狭窄患者肝内胆管组织(狭窄组)和40例肝内胆管结石仅合并慢性炎症患者肝内胆管组织(炎症组)及40例正常肝内胆管组织(正常组)中TGFβ1、Survivin和Caspase-3三种标志物的表达,比较各组三种标志物表达的差异,并分析三种标志物表达与肝内胆管结石相关胆管狭窄患者临床病理特征的关系。结果TGFβ1阳性表达率分别为狭窄组72.0%、炎症组37.5%、正常组15.0%,各组间差异均有统计学意义(均P<0.05);Survivin阳性表达率分别为狭窄组78.0%、炎症组47.5%、正常组25.0%,各组间差异均有统计学意义(均P<0.05);Caspase-3阳性表达率分别为狭窄组10.0%、炎症组42.5%、正常组75.0%,各组间差异均有统计学意义(均P<0.05)。肝内胆管结石相关胆管狭窄患者胆管组织中TGFβ1表达与Caspase-3蛋白呈显著负相关(r=-0.882,P<0.05),与Survivin呈显著正相关(r=0.889,P<0.05),Survivin与Caspase-3呈显著负相关(r=-0.923,P<0.05)。结论TGFβ1、Survivin和Caspase-3蛋白的异常表达,可能共同参与肝内胆管结石相关胆管狭窄形成的过程。Objective To investigate the expressions and the significance among the three markers TGF β1, Survivin and Caspase-3 in intrahepatic bile duct tissues in patients with intrahepatic bile duct stones. Method Total of 130 paraffin section of intrahepatic bile duct tissue were collected at Department of Pathology, The 904th Hospital of Joint Logistic Support Force of PLA from 2013 to 2018. Total of 50 patients with intrahepatic bile duct stones complicated with bile duct strictures (the stenosis group), 40 patients with intrahepatic bile duct stones with chronic inflammation (the inflammation group), and 40 patients with normal liver tissues (the normal control group) were included in this study. The expressions of TGF β1, Survivin and Caspase-3 in liver tissues were detected by immunohistochemistry and compared among the 3 groups to find their correlations with the clinicopathological features of the disease of the patients. Results TGF β1 was expressed in 72.0% of the patients in the stenosis group, 37.5% in the inflammatory group, and 15.0% in the normal control group. The differences among the groups were significant (P<0.05);Survivin was expressed in 78.0% of the patients in the stenosis group, 47.5% in the inflammatory group, and 25.0% in the normal control group. The differences among the groups were significant (P<0.05);Caspase-3 was expressed in 10.0% of the patients in the stenosis group, 42.5% in the inflammatory group, and 75.0% in the normal control group. The differences among the groups were significant (P<0.05). Within the stenosis group, TGF β1 was negatively correlated with Caspase-3 (r=-0.882, P<0.05), and positively correlated with Survivin (r=0.889, P<0.05). Survivin and Caspase-3 were also negatively correlated (r=-0.923, P<0.05). Conclusion Abnormal expressions of TGF β1, Survivin and Caspase-3 were involved in the formation of intrahepatic bile duct stones associated with bile duct strictures.

关 键 词：转化生长因子Β1 半胱氨酸天冬氨酸蛋白酶-3 生存素 肝内胆管结石 胆管狭窄 

分 类 号：R575.7[医药卫生—消化系统]