苏丹Ⅳ与DNA在含金属离子环境中相互作用的研究  

Study on the Interation between Sudan Ⅳ and DNA in the Presence of Metal Ions

作　　者：付彩霞[1] 荣先国[1] 高宗华[1] FU Cai-xia;RONG Xian-guo;GAO Zong-hua(Chemistry Staff Room,Binzhou Medical College,Yantai 264003,China)

机构地区：[1]滨州医学院化学教研室

出　　处：《安徽化工》2019年第3期48-50,共3页Anhui Chemical Industry

摘　　要：用荧光和紫外光谱法研究了苏丹Ⅳ与DNA在含金属离子环境中的相互作用.结果表明:DNA及金属离子均导致苏丹Ⅳ的内源荧光猝灭,猝灭机制均为静态猝灭.运用Scatchard方程对实验数据进行处理分析,求得在有无金属离子存在下苏丹Ⅳ与DNA相互作用的结合常数和结合位点数.讨论了金属离子对苏丹Ⅳ与DNA相互作用的影响.The interation between Sudan Ⅳ and herring sperm DNA in the presence of metal ions was investigated by fluorescence and ultraviolet spectroscopy. The results showed that both DNA and metal ions cause the quench of the fluorescence intensity of Sudan Ⅳ in a static quenching mechanism. The binding constants and sites in the absence and presence of metal ions were obtained by scatchard equation . The effects of metal ions on the interaction of Sudan Ⅳ with DNA were also discussed.

关 键 词：苏丹Ⅳ 鲱鱼精DNA 金属离子 光谱法 影响 

分 类 号：O657.3[理学—分析化学]