转化生长因子β1对肺成纤维细胞中平滑肌肌动蛋白表达的影响  

Effect of Transforming Growth Factor β1 on the Expression of Smooth Muscle Actin in Lung Fibroblasts

作　　者：孙林清 周倩文 王发选 SUN Linqing;ZHOU Qianwen;WANG Faxuan(School of Public Health and Management,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学公共卫生与管理学院

出　　处：《宁夏医科大学学报》2019年第5期461-464,共4页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81660534)

摘　　要：目的观察不同浓度转化生长因子β1(transforming growth factorβ1,TGF-β1)刺激人胚肺成纤维细胞(MRC-5)后对细胞内平滑肌肌动蛋白(alpha smooth muscle actin,α-SMA)表达的影响。方法以浓度为0、2.5、5.0和10.0ng·mL^-1的TGF-β1刺激后收集细胞,采用实时荧光定量聚合酶链反应(Quantitative Real-time PCR)检测MRC-5细胞内α-SMA基因的相对表达水平,Western blot法测定MRC-5细胞内α-SMA蛋白的相对表达水平,采用Spearman秩相关分析TGF-β1剂量与MRC-5细胞中α-SMA基因和蛋白的相对表达量之间的相关性。结果随着TGF-β1剂量的增加,各组MRC-5细胞中α-SMA基因表达水平增高,5.0ng·mL^-1和10.0ng·mL^-1剂量组高于0ng·mL^-1组和2.5ng·mL^-1组(P均<0.05);10.0ng·mL-1剂量组高于5.0ng·mL^-1(P=0.004)。5.0ng·mL^-1组和10.0ng·mL^-1组MRC-5细胞中的α-SMA蛋白相对表达量高于0ng·mL^-1组(P均<0.05)。TGF-β1剂量与α-SMA基因和蛋白间均呈正相关关系(r基因=0.972,r蛋白=0.878;P基因=0.000,P蛋白=0.042)。结论TGF-β1能影响肺成纤维细胞中α-SMA的表达情况。Objective The effects of different concentrations of transforming growth factorβ1(TGF-β1)on the expression of alpha smooth muscle actin(α-SMA)in human embryonic lung fibroblasts(MRC-5)were observed.Methods Cells were collected after stimulation with TGF-β1 at a concentration of 0ng·mL-1,2.5ng·mL^-1,5.0ng·mL^-1,and 10.0ng·mL^-1.Quantitative real-time PCR was used to detect the relative expression levels ofα-SMA gene in MRC-5 cells,and Western blot was used to determine the relative expression levels ofα-SMA protein.Correlation between the dose of TGF-β1 and the relative expression ofα-SMA gene and protein in MRC-5 cells was analyzed by spearman rank correlation analysis.Results With the increasing of TGF-β1 stimulation dose,the expression level ofα-SMA gene in each group of MRC-5 cells was increased,and the dose groups of 5.0 ng·mL^-1 and 10.0ng·mL^-1 were higher than those of 0ng·mL^-1 group and 2.5ng·mL^-1 group(P all<0.05);10.0ng·mL^-1 dose group was higher than 5.0ng·mL^-1(P=0.004).The relative expression levels ofα-SMA protein in 5.0ng·mL-1 group and 10.0ng mL-1 group treated MRC-5 cells were higher than 0ng·mL^-1 group(P all<0.05).And the dose of TGF-β1 was positively correlated withα-SMA gene and protein expression levels(rgene=0.972,rprotein=0.878;Pgene=0.000,Pprotein=0.042).Conclusion TGF-β1 can affect the expression ofα-SMA in lung fibroblast cells.

关 键 词：转化生长因子Β1 肺纤维化 平滑肌肌动蛋白α-SMA 肺成纤维细胞 

分 类 号：R135[医药卫生—劳动卫生]