丹参酮ⅡA对大鼠心肌梗死后心房颤动易感性的影响  

The Influence of Tanshinone ⅡA on Post-myocardial Infarction Atrial Fibrillation in Rats

作　　者：马世玉 马金 丁春华 张敏州 MA Shiyu;MA Jin;DING Chunhua;ZHANG Minzhou(Second Affiliated Hospital of Guangdong University of Chinese Medicine,Guangzhou 510006,Guangdong,China;Guangzhou Key Laboratory of Chinese Medicine for Prevention and Treatment of Myocardial Infarction)

机构地区：[1]广州中医药大学第二附属医院,广州510006 [2]广州市心肌梗死中医药防治重点实验室

出　　处：《中西医结合心脑血管病杂志》2019年第12期1789-1794,共6页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：广东省自然科学基金(No.2017A030313888,2016A030313634);广东省中医院中医药科学技术研究专项(No.YN2018MJ02,YN2015MS10)

摘　　要：目的观察丹参酮ⅡA对大鼠心肌梗死后心房颤动诱发率及持续时间的影响,并初步探讨其作用机制。方法将大鼠分为假手术组、心肌梗死组、丹参酮ⅡA组,大鼠心肌梗死后1周开始给予丹参酮ⅡA灌胃4周。采用经食管高频起搏左房技术检测心房颤动诱发率和持续时间,Masson染色和羟脯氨酸检测左房总胶原蛋白含量,蛋白免疫印迹法检测左心房Ⅰ型和Ⅲ型胶原蛋白含量并检测纤维化相关因子基质金属蛋白酶-9(MMP-9)/金属蛋白酶组织抑制剂1(TIMP-1)变化。结果丹参酮ⅡA组给药4周后,心房颤动诱发率低于心肌梗死组,持续时间显著短于心肌梗死组,差异均有统计学意义(P<0.05)。与心肌梗死组比较,丹参酮ⅡA组左心房心肌纤维化面积百分比降低,Ⅰ型和Ⅲ型胶原蛋白含量降低,MMP-9蛋白表达降低,TIMP-1蛋白表达升高,MMP-9/TIMP-1比值降低,差异均有统计学意义(P<0.05)。结论丹参酮ⅡA通过调节MMP-9/TIMP-1平衡抑制左心房纤维化,从而降低心肌梗死大鼠心房颤动发生率和持续时间。Objective To investigate the influence of tanshinone Ⅱa on post-myocardial infarction(MI)atrial fibrillation(AF) in rats.Methods The rats were divided into Sham group,MI group,and tanshinone ⅡA group.After MI one week,tanshinone ⅡA was gavaged once a day for 4 weeks.AF inducibility and duration were detected by transesophageal programmed electrical stimulation AF inducing technology.Sirius red-fast green counter staining and hydroxyproline were used to detect the content of collagen in left atrium.The expression of Ⅰ and Ⅲ collagen and the changing of matrix metalloprotein 9(MMP-9)/metalloproteinase tissue inhibitor 1(TIMP-1) balance in left atrial were measured by Western blotting.Results After 4 weeks,the AF inducibility in the tanshinoneⅡA group was lower than that in the MI group( P <0.05),and the AF duration was reduced( P <0.05).Compared with MI group,percentage of myocardial fibrosis,Ⅰcollagen,and Ⅲ collagen in left atrium were decreased in the tanshinoneⅡA group( P <0.05).MMP-9 was lower in the tanshinoneⅡA group than that in the MI group.TIMP-1 was higher in the tanshinoneⅡA group than that in MI group,MMP-9/TIMP-1 ratio was decreased.Conclusion Tanshinone ⅡA reduces the inducibility and duration of AF after MI by inhibiting left atrial fibrosis via regulating MMP-9/TIMP-1 balance.

关 键 词：心肌梗死 心房颤动 大鼠 心肌纤维化 电生理 基质金属蛋白酶-9 金属蛋白酶组织抑制剂1 

分 类 号：R542.2[医药卫生—心血管疾病] R256.2[医药卫生—内科学]