一测多评法同时测定苏黄止咳胶囊中7种成分  

Simultaneous determination of seven constituents in Suhuang Zhike Capsules by QAMS

作　　者：王加良 张艳丽 范景辉 李海燕 WANG Jia-liang;ZHANG Yan-li;FAN Jing-hui;LI Hai-yan(Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang 157011, China)

机构地区：[1]牡丹江医学院附属红旗医院

出　　处：《中成药》2019年第7期1505-1510,共6页Chinese Traditional Patent Medicine

基　　金：牡丹江市科学技术计划项目(z2015s0023)

摘　　要：目的建立一测多评法同时测定苏黄止咳胶囊(牛蒡子、紫苏叶、紫苏子等)中牛蒡子苷、牛蒡子苷元、迷迭香酸、白花前胡甲素、白花前胡乙素、白花前胡E素、五味子醇甲的含有量。方法该药物甲醇提取液的分析采用Hypersil ODS C18色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-0.1%冰醋酸,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长250、280、321 nm。以迷迭香酸为内标,计算其他6种成分的相对校正因子,测定其含有量。结果 7种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.91%~100.1%,RSD 0.61%~1.40%。一测多评法所得结果与外标法接近。结论该方法稳定可靠,可用于苏黄止咳胶囊的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS) method for the simultaneous content determination of arctiin, arctigenin, rosmarinic acid, praeruptorin A, praeruptorin B, praeruptorin E and schisandrin in Suhuang Zhike Capsules(Arctii Fructus, Perillae Folium, Perillae Fructus, etc.).METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ thermostatic Hypersil ODS C18 column(4.6 mm×250 mm, 5 μm),with the mobile phase comprising of methanol-0.1% glacial acetic acid flowing at 1.0 mL/minin a gradient elution manner, and the detection wavelengths were set at 250, 280, 321 nm. Rosmarinic acid was used as an internal standard to calculate the relative correction factors of the other six constituents, after which the content determination was made.RESULTS Seven constituents showed good linear relationships within their own ranges(r>0.999 0), whose average recoveries were 96.91%-100.1% with the RSDs of 0.61%-1.40%. The results obtained by QAMS approximated those obtained by external standard method. CONCLUSION This stable and reliable method can be used for the quality control of Suhuang Zhike Capsules.

关 键 词：苏黄止咳胶囊 化学成分 一测多评 

分 类 号：R927.2[医药卫生—药学]