锁阳乙醇提取物对运动小鼠抗疲劳能力的影响  

Effect of ethanol extract of Cynomorium songaricum Rupr. on anti-fatigue ability in exercise mice

作　　者：万丽娜 王劼 杜玉枝[1] 孙菁[1] 孟卫东 卢学峰[1] 周玉碧[1] WAN Li-na;WANG Jie;DU Yu-zhi;SUN Jing;MENG Wei-dong;LU Xue-Feng;ZHOU Yu-bi(Key Laboratory of Tibetan Medicine Research and Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008;Graduate University of Chinese Academy of Sciences,Beijing 100049;Qinghai Daokang Agriculture and Animal Husbandry Technology Co Ltd,Dulan 816100,China)

机构地区：[1]中国科学院藏药研究重点实验室,中国科学院西北高原生物研究所,青海省青藏高原特色生物资源研究重点实验室,青海西宁810001 [2]中国科学院大学,北京100049 [3]青海道康农牧科技有限公司,青海都兰816100

出　　处：《甘肃农业大学学报》2019年第3期23-30,共8页Journal of Gansu Agricultural University

基　　金：青海省基础研究计划项目(2016-ZJ-765);青海省重点研发与转化项目(2019-SF-171);青海省重点实验室发展建设专项(2017-ZJ-Y10)

摘　　要：【目的】研究锁阳乙醇提取物对小鼠抗运动性疲劳作用的影响.【方法】选取青海产药材锁阳,通过加热回流提取获得锁阳乙醇提取物.将小鼠随机分为模型对照组,阳性对照组(红景天口服液、维生素E软胶囊),锁阳乙醇提取物低、中、高剂量组(0.125、0.25、0.5 g/mL),连续灌胃给药14 d后,记录小鼠爬杆时间、负重游泳时间及测定小鼠三磷酸腺苷(ATP)、肝糖原、肌糖原、血糖、血尿素氮(BUN)、血乳酸(LD)、丙二醛(MDA)、肌酸激酶(CK)生化指标.【结果】与模型对照组比较,锁阳乙醇提取物不同剂量组均能明显延长小鼠爬杆及负重游泳时间;显著增加ATP、肝糖原、肌糖原、血糖含量;显著减少CK、LD、BUN含量;锁阳乙醇提取物中、高剂量组显著减少MDA含量( P <0.05).【结论】在一定剂量范围内,锁阳乙醇提取物可以增强运动耐力、提高氧化应激能力和改善能量代谢,有显著的抗疲劳作用.【Objective】 To observe the effect of ethanol extract of C.songaricum on anti-fatigue in mice.【Method】 The herbal medicine of C.songaricum in Qinghai Province was selected and the extract was extracted by refluxing with ethanol.The mice were randomly divided into blank control group,positive control group ( Rhodiola oral liquid,vitamin E soft capsule),ethanol extract of C.songaricum high-dose,middle-dose,low-dose group (0.5,0.25,0.125 g/mL).After 14 days of continuous intragastric administration,the mice were recorded for climbing time,weight-bearing swimming time and determination of adenosine triphosphate (ATP),hepatic glycogen,muscle glycogen,blood glucose,blood urea nitrogen (BUN),blood lactic acid (LD),malondialdehyde (MDA),creatine kinase (CK).【Result】 Compared with the blank control group,the different doses of ethanol extract of C.songaricum significantly prolonged the time of climbing rod and weight-bearing swimming ( P <0.01);significantly increased ATP,hepatic glycogen,muscle glycogen,and blood glucose levels ( P <0.01);and significantly reduced CK,LD and BUN levels ( P < 0.01, P <0.05) in mouse;MDA content in mice was significantly decreased in medium and high dose groups of ethanol extract of C.songaricum .【Conclusion】 In a certain dose range,ethanol extract of C.songaricum has significant anti-fatigue effect by enhancing exercise tolerance,improving oxidative stress and improving energy metabolism.

关 键 词：锁阳 乙醇提取物 抗疲劳 

分 类 号：R965.1[医药卫生—药理学]