光干涉生物亲和性检测系统设计  

Design of bioaffinity detection system based on optical interference

作　　者：张硕 嵇晓强[1] 常知强 史健松 李琦[1] 张昊[1] 李美萱 ZHANG Shuo;JI Xiao-qiang;CHANG Zhi-qiang;SHI Jian-song;LI Qi;ZHANG Hao;LI Mei-xuan(School of Life Science and Technology,Changchun University of Science and Technology,Changchun130022,China;Institute for Interdisciplinary Quantum Information Technology,Jilin Engineering Normal University,Changchun130052,China;Jilin Engineering Laboratory for Quantum Information Technology,Changchun130052,China)

机构地区：[1]长春理工大学生命科学技术学院,吉林长春130022 [2]吉林工程技术师范学院量子信息技术交叉学科研究院,吉林长春130052 [3]吉林省量子信息技术工程实验室,吉林长春130052

出　　处：《光学精密工程》2019年第6期1378-1386,共9页Optics and Precision Engineering

基　　金：吉林省科技发展计划资助项目(No.20170309003YY);吉林省教育厅计划资助项目(No.JJKH20181142KJ)

摘　　要：分子互作检测是目前食品安全检测、临床癌症病理筛查等检测领域的研究热点。为了对生物分子间相互作用进行实时、快速检测,本文以光干涉技术为理论基础,设计了基于光纤生物传感器的分子互作检测系统。采用了石英光纤与自聚焦透镜耦合结构,提高了光源与干涉光的传输光路耦合效率。采用STM32微处理器作为主控制器,实现了数据采集、三维传动机构控制、恒温震荡控制、通讯等功能。最后,采用所设计的分子互作检测系统及美国Fortebio公司的BLItz分子互作检测系统,分别进行不同浓度的定量检测实验,实时检测IgG与抗原分子蛋白A的结合与解离反应。实验结果表明:本文设计的系统检测极限达到10μg/mL,重复性CV值小于6%,与BLItz接近。所设计系统检测的自动化程度高、检测过程中无需清洗、不产生交叉污染、成本低,能够满足药代动力学研究需要。Molecular interaction detection is a hot topic in the fields of food safety detection,clinical cancer pathological screening,and other areas of research. To realize real-time and rapid detection of interactions between biomolecules,a molecular interaction detection system based on a fiber biosensor was designed in this study. The coupling structure of a self-focusing lens and quartz fiber was adopted to improve the coupling efficiency of optical transmission between the light source and interference light. A STM32 microprocessor was used as the main controller to realize data acquisition,3D transmission mechanism control,constant temperature oscillation control,and other functions. Finally,the designed and BLItz molecular interaction detection systems were used to detect the different concentrations of immunoglobulin IgG and protein A,respectively. The binding and dissociation reactions of the IgG molecule and antigen molecule protein A were detected in real time. The experimental results show that the detection limit of the system could reach10 μg/mL,and the repeatability CV value was less than 6%,which was close to that of the BLItz. The designed system has the advantages of high automation,no need for cleaning,no cross contamination in the detection process,and low cost. Therefore,it can meet the needs of pharmacokinetics research.

关 键 词：分子亲和性检测 光干涉 光纤生物传感器 光纤耦合结构 STM32 免疫球蛋白IGG 

分 类 号：TH741[机械工程—光学工程]