柳珊瑚Leptogorgia rigida中甾体类成分研究  

Research on Sterols from Leptogorgia rigida

作　　者：肖细姬[1] 邓芸[1] 谢路凤[1] 张琪 韦霞 于鑫 张翠仙[1] XIAO Xiji;DENG Yun;XIE Lufeng;ZHANG Qi;WEI Xia;YU Xin;ZHANG Cuixian(School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou,510006,China)

机构地区：[1]广州中医药大学中药学院

出　　处：《热带海洋学报》2019年第4期64-69,共6页Journal of Tropical Oceanography

基　　金：国家自然科学基金项目(81741160);广东省科技计划(2015A020216017、2017A020217008);广州中医药大学“薪火计划”重点项目(XH20170110);海南大学南海海洋资源利用国家重点实验室开放课题(2016003);广东省自然科学基金项目(2014A030313411)~~

摘　　要：首次对南海柳珊瑚Leptogorgia rigida中甾体类次生代谢产物进行研究。采用硅胶柱柱层析、十八烷基硅烷键合硅胶填料、SephadexLH-20、高效液相色谱(HPLC)及薄层色谱(TLC)等多种方法进行分离纯化,通过核磁共振波谱(NMR)、质谱(MS)等现代波谱分析及物理常数对照等方法进行结构鉴定。从南海柳珊瑚Leptogorgia rigida次生代谢产物中分离得到8个甾类化合物,结构依次为:3β,6β,11-trihydroxy-9,11-seco-5α-chloest-7-en-9-one(1);3β,6α,11-trihydroxy-9,11-seco-5α-chloest-7-en-9-one(2);3β,6α,11-trihydroxy-5α-chloest-7,22(E)-dien-9-one(3);pregnan-4-en-3,20-dione(4);5α-pregnan-3,20-dione(5);3β-hydroxy-pregnan-5-en-20-dion(6);3β-hydroxy-5α-pregnan-20-one(7);和3β,6α, 11-trihydroxy-9, 11-seco-5α-gorgosterol (8)。所有化合物均首次从Leptogorgia属中得到。Research was carried out on novel secondary metabolites from Leptogorgia rigida collected from the South China Sea.The secondary metabolites were isolated by silica gel column chromatography,ODS,Sephadex LH-20,thin layer chromatography,and high performance liquid chromatography(HPLC).The structures of compounds were identified by nuclear magnetic resonance spectrum(NMR),mass spectrum(MS),and literature analysis.Eight sterols were obtained and elucidated as follows: 3β,6β,11-trihydroxy-24-methyl-5α-chloest-9,11-seco-7-en-9-one(1);3β,6α,11-trihydroxy-24-methyl-5α-chloest-9,11-seco-7-en-9-one(2);3β,6α,11-tri-hydroxy-5α-chloest-9,11-seco-7,22(E)-dien-9-one(3);pregnan-4-en-3,20-dione(4);5α-pregnan-3,20-dione(5);3β-hydroxy-pregnan-5-en-20-dione(6);3β-hydroxy-5α-pregnan-20-one(7);and Gorgosterol(8).All the sterols were first obtained from Leptogorgia rigida.

关 键 词：南海柳珊瑚Leptogorgia rigida 甾类化合物 断甾醇 结构鉴定 

分 类 号：O629.7[理学—有机化学] P735[理学—化学]