葛根素通过抑制脊髓胶质细胞活化和炎症反应治疗腰椎间盘突出症引起的神经根性疼痛（英文）  

Puerarin alleviates radicular pain caused by lumbar disc herniation by inhibiting spinal glial cell activation and inflammatory response

作　　者：胡育铭 朱丽蓉 赵元淑 许明贤 钟祎 HU Yu-ming;ZHU Li-rong;ZHAO Yuan-shu;XU Ming-xian;ZHONG Yi(Key Laboratory of Neuroscience, School of Basic Medical Science, Guangzhou Medical University, Guangzhou 511436 ,China)

机构地区：[1]广州医科大学基础医学院神经科学重点实验室

出　　处：《中国病理生理杂志》2019年第7期1316-1322,共7页Chinese Journal of Pathophysiology

基　　金：Supported by the National Natural Science Foundation of China(No.81600968);Medical Scientific Research Foundation of Guangdong Province of China(No.A2017101)

摘　　要：目的:从脊髓胶质细胞活化和炎症反应角度探究中药葛根素在治疗大鼠神经根性疼痛中的作用及机制。方法:用自体髓核(NP)移植方法复制大鼠腰椎间盘突出症模型,假手术组行同样手术但不进行NP移植。治疗组大鼠术前1 h开始腹腔注射不同浓度(50、100和150 mg/kg)葛根素注射液,每天1次,连续7 d。机械性缩爪阈值(PWT)检测法评价大鼠痛行为。免疫荧光染色法检测小胶质细胞和星形胶质细胞活化情况。ELISA检测脊髓抗炎和致炎细胞因子的含量。结果:与假手术组相比,NP移植后3~14 d,大鼠的PWT明显下降。与溶剂对照组相比,100 mg/kg和150 mg/kg的葛根素明显增加NP组大鼠的PWT,缓解疼痛症状。葛根素明显降低小胶质细胞标志物离子钙结合接头分子1和星形细胞标志物胶质细胞原纤维酸性蛋白的表达,并且降低致炎细胞因子肿瘤坏死因子α、白细胞介素1β(IL-1β)和IL-6的含量,增加抗炎细胞因子IL-10的含量(P <0. 01)。结论:葛根素通过抑制脊髓胶质细胞活化和炎症反应治疗腰椎间盘突出症诱导的神经根性疼痛。AIM: To investigate the role of Chinese medical herb Radix Puerariae extract puerarin in a rat model of radicular pain caused by lumbar disc herniation( RAPLDH) and to explore the possible mechanism involving spinal glial cell activation and inflammatory response. METHODS: The rat model of RAPLDH was induced by autologous nucleus pulposus( NP) implantation. The rats in sham group received the same operation procedure except NP implantation. Puerarin injection at different doses( 50,100 and 150 mg/kg) was delivered intraperitoneally 1 h before surgery,and once daily for7 d. Mechanical paw withdrawal threshold( PWT) test was employed for assessing pain behaviors. Spinal microglia and astrocyte activation was evaluated by immunofluorescence staining of relevant specific markers. The expression of pro-and antiinflammatory cytokines in spinal dorsal horn was measured by ELISA. RESULTS: The rats with NP implantation showed long-lasting pain behaviors,characterized by decrease in PWT from day 3 to day 14 after surgery. Compared with vehicle group,puerarin at doses of 100 mg/kg and 150 mg/kg significantly increased PWT of the rats with NP implantation. Puerarin significantly reduced the expression of spinal microglia marker ionized calcium-binding adaptor molecule 1 and astrocyte marker glial fibrillary acidic protein( P < 0. 01). Puerarin also decreased spinal expression of pro-inflammatory cytokines tumor necrosis factor-α,interleukin-1β and interleukin-6,and increased anti-inflammatory cytokine interleukin-10( P <0. 01). CONCLUSION: Puerarin alleviate RAPLDH by inhibiting spinal glial cell activation and inflammatory response.

关 键 词：葛根素 神经根性疼痛 腰椎间盘突出症 脊髓胶质细胞 炎症反应 

分 类 号：R681.53[医药卫生—骨科学] R363.2[医药卫生—外科学]