Linc00152在骨肉瘤中的表达及其与细胞增殖、迁移的关系  

Expression and biological function of cell proliferation and migration of linc00152 in human osteosarcoma

作　　者：杜文君[1] 张雪松[2] 孙娟[1] 夏英鹏[1] 朱如森[1] 张诗武[3] Du Wenjun;Zhang Xuesong;Sun Juan;Xia Yingpeng;Zhu Rusen;Zhang Shiwu(Department of Spine Center,Tianjin Union Medical Center,Nankai University Affiliated Hospital,Tianjin300121,China;Department of Orthopaedics,Tangshan Second Hospital,Tangshan Orthopaedic Hospital,Tangshan 063608,China;Department of Pathology,Tianjin Union Medical Center,Nankai University AffiliatedHospital,Tianjin 300121,China)

机构地区：[1]天津市人民医院南开大学人民医院脊柱中心,300121 [2]唐山市第二医院唐山市骨科医院,063608 [3]天津市人民医院病理科,300121

出　　处：《中华实验外科杂志》2019年第7期1253-1255,共3页Chinese Journal of Experimental Surgery

基　　金：天津市人民医院科技基金项目(2017YJ007).

摘　　要：目的探讨长链非编码RNA(lncRNA) linc00152在骨肉瘤中的表达及其与细胞增殖迁移的关系.方法采用实时荧光定量聚合酶链反应(Real-time PCR)技术检测天津市人民医院收治并手术治疗的26骨肉瘤患者癌组织、远癌的正常骨组织及SaoS2、U2OS、HOS、MG63骨肉瘤细胞株及正常骨细胞株hFOB1.19中linc00152的差达水平,分析linc00152在不同的组织及细胞株中的差异表达.应用小干扰RNA(siRNA)技术下调骨肉瘤细胞株中linc00152表达,比较下调前后骨肉瘤细胞增殖、迁移能力变化情况.应用SPSS 17.0统计软件分析,计量资料以均值±标准差(Mean±SD)表示,两组之间比较采用t检验.结果人骨肉瘤细胞(SaoS2、U2OS、HOS及MG63)中linc00152的相对表达水平分别为7.23 ±2.11,5.02±1.89,4.21 ±1.51和3.75±1.52,显著高于正常骨细胞株hFOB1.19(1.21±0.22)且差异有统计学意义(F=9.358,P<0.01).26例骨肉瘤患者中,肿瘤组织与正常骨组织中linc00152的相对表达量分别为4.12±1.81和1.97±1.03,肿瘤组织显著高于正常组织,差异有统计学意义(t=5.490,P<0.01).在人骨肉瘤细胞SaoS2细胞内转染针对linc00152的小干扰RNA (si_00152)及无关序列(si_nc),发现SaoS2细胞下调linc00152表达后,其增殖能力、迁移能力及穿膜能力显著降低.结论lncRNA linc00152在入骨肉瘤组织及细胞株中表达明显增高,并参与了细胞增殖及迁移生物学过程.Objective To explore the expression and biological function of cell proliferation and migration of linc00152 in human osteosarcoma. MethodsReal-time PCR was used to detect the differential expression of linc00152 in cancer tissue, normal bone tissue and SaoS2, U2OS, HOS, MG63 osteosarcoma cell lines and normal bone hFOB1.19 cell line. The expression of linc00152 in osteosarcoma cell lines was downregulated by small interfering RNA (siRNA), and the proliferation and migration ability of osteosarcoma cells was evaluated after knocking down linc00152 expression. ResultsThe linc00152 relative expression in SaoS2, U2OS, HOS and MG63 osteosarcoma cells were 7.23±2.11, 5.02±1.89, 4.21±1.51 and 3.75±1.52 which was significant higher than normal bone cells hFOB1.19 (1.21±0.22) with statistical difference (F=9.358, P<0.01);line00152 relative expression in cancer tissue and normal tissue were 4.12±1.81 and 1.97±1.03 respectively with statistical difference (t=5.490, P<0.01);The small interference RNA (si_00152) and unrelated sequences (si_nc) were transfected into the human osteosarcoma SaoS2 cells. It was found that the proliferation ability, migration ability and trans membrane ability of SaoS2 cells decreased significantly after down regulated linc00152 expression in SaoS2 cells. ConclusionLinc00152 increased significantly in human osteosarcoma tissues and cell lines, and participated in the biological process of cell proliferation and migration.

关 键 词：骨肉瘤 linc00152 增殖 迁移 

分 类 号：R738.1[医药卫生—肿瘤]