淫羊藿苷对脂多糖诱导成骨细胞骨架F-actin损伤的保护作用  

Protective Effect of Icariin on Lipopolysaccharide-induced Cytoskeleton F-actin Injury

作　　者：黄孝闻[1] 王绪平[1] 张扬[1] 寿旦[1] HUANG Xiaowen;WANG Xuping;ZHANG Yang;SHOU Dan(Zhejiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China)

机构地区：[1]浙江省中医药研究院

出　　处：《中国现代应用药学》2019年第13期1612-1616,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81873062,81803808);浙江省中医药科技计划项目(2016ZB003,2018ZA021);浙江省科技计划项目(2017F30047)

摘　　要：目的考察淫羊藿苷对成骨细胞骨架F-actin损伤的保护作用及其对相关信号通路的调控。方法采用脂多糖诱导大鼠成骨细胞损伤模型。实验分为空白对照组、模型组和0.1,1,10μg·mL^-1淫羊藿苷组,MTT法观察淫羊藿苷对成骨细胞活性的影响,TRITC-Phalloidin荧光染色观察淫羊藿苷对细胞骨架的影响,ELISA法测定各组成骨细胞中F-actin的含量,RT-PCR法测定细胞骨架RhoA和Cofilin的mRNA表达量。结果与空白对照组比较,100μg·mL^-1脂多糖能显著降低成骨细胞存活率(P<0.01);与模型组比较,1~10μg·mL^-1淫羊藿苷预处理后能显著提高细胞存活率(P<0.05或P<0.01);而0.1μg·mL^-1淫羊藿苷则无显著影响。与模型组比较,1~10μg·mL^-1淫羊藿苷能显著抑制成骨细胞F-actin解聚(P<0.05),抑制RhoA的mRNA表达(P<0.05或P<0.01),0.1~10μg·mL^-1淫羊藿苷能抑制Cofilin的mRNA表达(P<0.01)。结论淫羊藿苷对脂多糖诱导的细胞骨架F-actin损伤具有保护作用,其机制与抑制细胞骨架相关因子RhoA和Cofilin的mRNA表达有关。OBJECTIVE To investigate the protective effect of icariin on F-actin damage of osteoblast cytoskeleton and the regulation of related signaling pathways.METHODS Lipopolysaccharide(LPS) was used to induce osteoblasts to establish an injury model in vitro.Experimental group: control group,model group and 0.1,1,10 μg·mL^-1 icariin groups.MTT method was used to observe the effect of icariin on the activity of osteocytes.TRITC-Phalloidin fluorescence staining was used to observe the effect of icariin on cytoskeleton.ELISA method was used to determine the content of F-actin in osteocytes.RT-PCR method was used to determine the expression of cytoskeleton-related factors Rho A and Cofilin mRNA.RESULTS Compared with the control group,100 μg·mL^-1 LPS could significantly reduce the survival rate of osteoblasts(P<0.01).Compared with the model group,the survival rate could significantly improve after pretreatment with 1-10 μg·mL^-1 icariin(P<0.05 or P<0.01),while 0.1 μg·mL^-1 icariin had no significant effect.Compared with the model group,1-10 μg·mL^-1 icariin could significantly inhibit F-actin depolymerization in osteoblasts(P<0.05),inhibit the mRNA expression of Rho A (P<0.05 or 0.01),and icariin at concentration of 0.1-10 μg·mL^-1 could inhibit the mRNA expression of Cofilin (P<0.01).CONCLUSION Icariin has protective effect on LPS-induced cytoskeleton F-actin injury model,and its mechanism may be related to inhibiting the expression of cytoskeleton-related factors Rho A and Cofilin mRNA.

关 键 词：淫羊藿苷 脂多糖 细胞骨架 F-肌动蛋白 

分 类 号：R285.5[医药卫生—中药学]