虫草多糖对平阳霉素诱导的肺纤维化小鼠的影响  

Effect of Cordyceps Polysaccharide on Pingyangmycin Induced Pulmonary Fibrosis in Mice

作　　者：扈荣 杨悦 白海波 傅旭春[3] HU Rong;YANG Yue;BAI Haibo;FU Xuchun(Hangzhou Huadong Medicine Group Pharmaceutial Research Institute,Hangzhou 310011,China;Hangzhou Zhongmei Huadong Pharmaceutical Co.,Ltd.,Hangzhou 310011,China;Zhejiang University City College,Hangzhou 310015,China)

机构地区：[1]杭州华东医药集团新药研究院有限公司,杭州310011 [2]杭州中美华东制药有限公司,杭州310011 [3]浙江大学城市学院,杭州310015

出　　处：《中国现代应用药学》2019年第13期1639-1642,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的研究虫草多糖抗小鼠肺纤维化的作用。方法采用气管注射平阳霉素制作小鼠肺纤维化模型,酶联免疫吸附法测定血清白介素1 受体抑制剂(interleukin-1 receptor antagonist,IL-1RA)含量,碱水解法测定肺组织中羟脯氨酸(hydroxyproline,Hyp)含量,Masson 染色法观察肺组织胶原沉积情况。结果与肺纤维化模型组相比,虫草菌粉组、虫草总多糖组和虫草多糖[分子量(molecular mass,MW)<2×10^5]组血清中IL-1RA 水平明显升高,肺组织中Hyp 含量明显降低;虫草总多糖组和虫草多糖(MW<2×10^5)组肺组织纤维化面积明显减小。结论虫草多糖具有明显的抗小鼠肺纤维化作用。OBJECTIVE To study the effects of cordyceps polysaccharide on pulmonary fibrosis model of mice. METHODS The pulmonary fibrosis model of mice was established by intratracheal injection of pingyangmycin.The content of interleukin-1 receptor antagonist(IL-1RA) in serum was determined by ELISA,and the content of hydroxyproline(Hyp) in lung tissue was determined by alkali hydrolysis.Collagen deposition in lung tissue was observed by Masson staining.RESULTS Compared with the pulmonary fibrosis model group,the content of IL-1RA in serum of fermentation cordyceps powder group, total cordyceps polysaccharide group and cordyceps polysaccharide group [molecular mass(MW)<2×10^5] evidently increased, but the content of Hyp in lung tissue evidently decreased.The fibrosis area of total cordyceps polysaccharide group and cordyceps polysaccharide group(MW<2×10^5) evidently decreased.CONCLUSION The cordyceps polysaccharide have significant anti-pulmonary fibrosis effects on model mice induced by pingyangmycin.

关 键 词：虫草多糖 发酵虫草菌粉 肺纤维化 MASSON染色 

分 类 号：R285.5[医药卫生—中药学]