猫人参含药血清对胃癌细胞SGC7901增殖和自噬的影响  

Effects of Medicinal Serum of Actinidia Valvata Dunn on Proliferation and Autophagy of Gastric Cancer Cell SGC7901

作　　者：王霞[1] 董孟佳 WANG Xia;DONG Mengjia(Jiangsu Second Hospital of TCM,Nanjing Jiangsu China 210017)

机构地区：[1]江苏省第二中医院

出　　处：《中医学报》2019年第7期1450-1453,共4页Acta Chinese Medicine

基　　金：江苏省自然科学基金项目(BK20161605);江苏省第二中医院院内科研基金项目(SEZJJP201604)

摘　　要：目的:观察猫人参对胃癌细胞SGC7901的增殖抑制作用及对自噬的影响。方法:体外培养胃癌SGC7901细胞,用不同体积分数的猫人参含药血清处理后,采用CCK-8法检测细胞增殖率,Western blot检测自噬相关蛋白LC3B、p62及mTOR表达水平。结果:猫人参含药血清作用24 h,对胃癌SGC7901细胞有明显的抑制作用,与空白血清组比较,猫人参含药血清组体积分数为0. 156 25%时,差异有统计学意义(P <0. 05),且随着体积分数的增大,抑制作用增强,呈剂量依赖性;作用48 h,与空白血清组比较,猫人参含药血清在体积分数<2. 5%时对胃癌SGC7901细胞有稍微促进增殖作用,在体积分数≥5%时有抑制增殖作用,体积分数≥10%时,差异开始有统计学意义(P <0. 05)。Western blot结果显示:与空白血清组比较,猫人参含药血清能促进胃癌SGC7901细胞LC3B蛋白表达,抑制p62、mTOR蛋白表达(P <0. 05)。结论:猫人参含药血清能抑制胃癌细胞SGC7901的增殖,其作用机制可能与猫人参能调节LC3B、p62、mTOR蛋白的表达,影响自噬相关。Objective: To observe the effects of Actinidia Valvata Dunn( AVD) on proliferation and autophagy of gastric cancer cell line SGC7901. Methods: SGC7901 cells of gastric cancer were cultured in vitro and treated with different volume fractions of serum containing AVD. The proliferation rate of SGC7901 cells was detected by CCK-8 method. The expression levels of autophagy-related proteins LC3B, p62 and mTOR were detected by Western blot. Results : Medicinal serum of AVD has obvious inhibitory effect on SGC7901 cells of gastric cancer for 24 hours. Compared with the blank serum group, when the volume fraction of AVD containing serum group was 0. 156 25%,the difference was statistically significant ( P <0. 05 ). With the increase of volume fraction,the in? hibitory effect increased, which was dose-dependent. After 48 hours, compared with the blank serum group, the serum containing AVD had slightly promoting effect on the proliferation of SGC7901 cells when the volume fraction was less than 2. 5%,and inhibiting effect when the volume fraction was more than 5 %. The difference was significant when the volume fraction was more than 10%(P < 0. 05). Western blot results showed that compared with the blank serum group, the serum containing AVD could promote the expression of LC3B protein and inhibit the expression of p62 and mTOR protein in SGC7901 cells (P <0. 05). Conclusion :Medicinal serum of AVD can inhibit the proliferation of gastric cancer cell SGC7901. Its mechanism may be related to the regulation of LC3B, p62 and mTOR protein expression by AVD and the influence of autophagy.

关 键 词：猫人参 胃癌细胞 增殖 自噬 

分 类 号：R285.5[医药卫生—中药学]