益胃汤延缓初老雌性SD大鼠皮肤衰老的研究  

Study of Yiwei Decoction on Delaying Skin Aging in Aging Female SD Rats

作　　者：张英杰[1] 刘岩 路芳 隋凯悦 ZHANG Yingjie;UU Yan;LU Fang;SUI Kaiyue(Shandong University of Chinese Medicine Jinan Shandong China 250000;Zibo Integration of Chinese andWestern Medicine Hospital,Zibo Shandong China 255000)

机构地区：[1]山东中医药大学,山东济南250000 [2]淄博市中西医结合医院,山东淄博255000

出　　处：《中医学报》2019年第7期1483-1487,共5页Acta Chinese Medicine

摘　　要：目的:探讨补益阳明津气方药益胃汤延缓初老雌性SD大鼠皮肤衰老的作用,并分析其机理。方法:根据月龄、阴道细胞学表现将SD雌性大鼠随机分为5组(n=10):正常对照组、初老对照组、初老实验组、去势对照组、去势实验组。对照组给予生理盐水灌胃,实验组给予益胃汤灌胃,灌胃8周后皮肤水分测试仪检测大鼠皮肤含水量和弹性。将大鼠处死,取大鼠背部皮肤组织匀浆,酶联免疫吸附实验检测I型胶原蛋白(Collagen 1,COL1)含量、超氧化物歧化酶(superoxide radical,SOD)活性、丙二醛(malonaldehyde,MDA)和羟脯氨酸(hydroxyproline,HYP)水平。结果:治疗后,实验组(初老实验组、去势实验组)的皮肤含水量明显高于对照组(初老对照组、去势对照组),差异均有统计学意义(P <0. 05);实验组的皮肤弹性优于对照组,差异有统计学意义(P <0. 05);实验组COL1、SOD、HYP含量明显高于对照组,实验组的MDA含量明显低于初老对照组,差异均有统计学意义(P <0. 01)。结论:补益阳明津气方药益胃汤可以增加大鼠皮肤含水量,改善皮肤弹性,增加皮肤SOD活性及降低MDA含量而提高皮肤组织抗氧化能力,增加HYP和COL1含量而提高皮肤弹性,具有延缓初老雌鼠皮肤衰老的作用。Objective: To explore the anti-aging effect of Yiwei Decoction of tonifying fluid and qi of Yangming prescription on the skin of aging female SD rats and analyze its mechanism. Methods: Female SD rats were randomly divided into 5 groups according to their age and vaginal cytology ( n = 10): normal control group, aging control group, aging experimental group, castrated control group and castrated experimental group. The control group was given normal saline and the experimental group was given Yiwei Decoction. After 8 weeks, the water content and elasticity of rat skin were monitored by skin moisture tester pen. Rats were sacrificed ,and the skin homogenate of the back was taken. The content of Collagen 1 ( COL1 ), the activity of superoxide radical (SOD),malonaldehyde ( MDA) and hydroxyproline ( HYP) levels were detected by enzyme-linked immunosorbent assay. Results :After treatment, the skin water content of the experimental group ( aging experimental group and castrated experimental group) was significantly higher than that of the control group ( aging control group and castrated control group), the difference was statistically significant ( P < 0. 05 );the skin elasticity of the experimental group was better than that of the control group, the difference was statistically significant (P <0. 05 );the COL1 ,SOD, HYP content of the experimental group was significantly higher than that of the control group, and the MDA content of the experimental group was significantly higher than that of the control group. The quantity was significantly lower than that of the control group ( P <0. 01 ). Conclusion: Yiwei Decoction of tonifying flu-id and qi of Yangming prescription can increase skin water content and improve skin elasticity, increase antioxidant capacity of skin tissues by increasing SOD activity and reducing MDA content, and increase skin elasticity by increasing the contents of HYP and COL 1. It has the effect of delaying skin aging of aging female rats.

关 键 词：补益阳明津气 益胃汤 皮肤衰老 大鼠 

分 类 号：R285.5[医药卫生—中药学]